The Effect of Glucose and Tolbutamide on Immunoreactive Somatostatin Release from Perifused Pancreatic Islets of Normal and Streptozotocin Diabetic Rats
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概要
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The patterns of immunoreactive somatostatin (IRS) responses to glucose and tolbutamide were investigated in perifused pancreatic islets of both normal and diabetic rats.<BR>The incubation chamber of the perifusion apparatus for the islets was devised with a 2.5 m<I>l</I> disposable syringe. By the method of Lacy, the pancreatic islets were isolated from diabetic rats about 30 days after a single intravenous injection of streptozotocin (STZ, 60 mg/kg body weight) and from normal rats of comparable body weight. The fasting blood glucose was 437±3 mg/100 m<I>l</I> in the diabetic rats and 120±5 mg/100 m<I>l</I> in normal ones. The concentration of IRS in each effluent was measured by the specific double antibody radioimmunoassay for somatostatin.<BR>During the initial 20 min incubation period with the medium containing 2.8 mM glucose, the levels of IRS were 11.3±1.0 pg/100 islets/2 min in normal islets and 32.0±3.4 pg/100 islets/2 min in diabetic islets. The latter value was significantly higher than the former (p<0.001). IRS levels gradually rose following 16.7 mM glucose and reached the maximal values with 30.2±5.7 pg/100 islets/2 min at 66 min in normal islets and with 56.8±13.3 pg/100 islets/2 min at 88 min in diabetic islets. The levels of IRS in diabetic islets were significantly higher than those of normal islets (p<0.001) throughout the experimental period. In the case of tolbutamide the IRS levels increased promptly to 51.9±6.6 pg/100 islets/2 min in diabetic islets and to 23.7±4.6 pg/100 islets/2 min in normal islets 2 min after initiation of the infusion. The former value was significantly higher than the latter (p<0.001). The levels of immunoreactive glucagon (IRG) in response to tolbutamide were significantly higher in the diabetic islets than in normal islets (p<0.001). These results indicate that there exists a hypersecrecretion of IRS from STZ-induced diabetic rats during the infusion with glucose and tolbutamide.
- 社団法人 日本内分泌学会の論文
著者
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SAWANO SHINJI
Department of Endocrinology and Metabolism, Toranomon Hospital
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KOKUBU TOMOKUNI
Research Institute for Polymers and Textiles
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Takeda Shigemasa
First Department Of Medicine Toho University Of School Of Medicine
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Kobayashi Tetsuro
Department Of Electrical Engineering Faculty Of Engineering Science Osaka University
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Kobayashi Tetsuro
First Department Of Medicine Toho University Of School Of Medicine
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ITOH TOKUJI
Department of Endocrinology and Metabolism, Toranomon Hospital, Okinaka Memorial Institute for Medic
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Sawano Shinji
Department Of Endocrinology And Metabolism Toranomon Hospital
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TAKEDA SHIGEMASA
First Department of Medicine, Toho University School of Medicine
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ITOH TOKUJI
Department of Endocrinology and Metabolism, Toranomon Hospital, Okinaka Memorial Institute for Medical Research and Asahi Life Institute for Adult Diseases
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