Effect of Synthetic Growth Hormone-Release Inhibiting Factor on the Secretion of Growth Hormone and Prolactin in Rats

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概要

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• The activities of the synthetic cyclized tetradecapeptide (GIF, Somatostatin) were investigated in isolated rat pituitaries <I>in vitro</I> and in rats treated with nembutal under ether anesthesia or with chlorpromazine (CPZ) under urethane anesthesia. It was found in <I>in vitro</I> study that 250nM or larger doses of the cyclized GIF partially but significantly inhibited GH release and that the incorporation of <SUP>14</SUP>C-leucine into the pituitary GH was also significantly reduced by 25nM of the cyclized, whose dose neither affected the release of synthesized GH into the medium nor suppressed the release of immunoreactive GH. On the other hand, a single intravenous (iv) injection of 100μg of the cyclized GIF did not affect the basal levels of both serum GH and prolactin. The raised levels of serum GH induced by nembutal (2.5mg/100g body weight, iv) were completely suppressed by a concomitant iv administration of 100μg of GIF but not 10μg. CPZ (500μg/100g body weight, iv)-induced serum GH rise was also completely inhibited by 100μg of GIF, whose dose did not suppress CPZ-induced serum prolactin rise. In another study, 5μg of GIF was given by a single iv injection with nembutal and 10μg of GIF was immediately infused for 30min. No increase in serum GH levels was observed in any rat during the infusion, but the levels were abruptly elevated in 7 out of 9 rats 15min after the cessation of the infusion. The similar ‘rebound’was noticed in 4 out of 9 rats when the dose of GIF was reduced to one fifth. The levels of serum GH were also abruptly raised at the same interval in 3 out of 7 rats given iv both CPZ (50μg/100g body weight) and GIF, but not in any rat treated with GIF alone.

• 社団法人 日本内分泌学会の論文

著者

• SAWANO SHINJI

  Department of Endocrinology and Metabolism, Toranomon Hospital

• ISHIZUKA YUSAKU

  Department of Chemistry, College of General Education, University of Tokyo

• BABA YOSHIHIKO

  Department of Endocrinology, Toranomon Hospital, Okinaka Memorial Institute for Medical Research, Sankyo Central Research Laboratories

• KOKUBU TOMOKUNI

  Department of Endocrinology, Toranomon Hospital, Okinaka Memorial Institute for Medical Research, Research Institute for Polymers and Textiles

• Sawano Shinji

  Department Of Endocrinology And Metabolism Toranomon Hospital

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• Effect of Theophylline on Insulin, Glucagon and Somatostation Secretion from the Isolated Rat Pancreatico-Duodenal Preparation
• Extraction and Partial Purification of Prolactin-Release Stimulating Factor in Bovine Hypothalami
• Effect of a Bovine Hypothalamic Factor on the Release and Biosythesis of Growth Hormone
• In vitro and and in vivo Studies on the Regulation of Somatostatin Release by Adrenergic Mechanism in Normal and Diabetic Rats
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• The Effect of Glucose and Tolbutamide on Immunoreactive Somatostatin Release from Perifused Pancreatic Islets of Normal and Streptozotocin Diabetic Rats
• Effect of Synthetic Growth Hormone-Release Inhibiting Factor on the Secretion of Growth Hormone and Prolactin in Rats
• Simultaneous Determinations of Plasma ACTH and GH Levels after an Oral Metyrapone

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