Preparation of chlorophyll-a and chlorophyll-b by column chromatography with Sephasorb HP Ultrafine.

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概要

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• A method for the preparation of chlorophyll-a (Chl-a) and chlorophyll-b (Chl-b) by column chromatography with Sephasorb HP Ultrafine has been developed. The partially purified chlorophyll preparation containing Chl-a, Chl-b and nonsorbed carotenes was applied to a Sephasorb HP Ultrafine column and good separation of Chl-a and Chl-b achieved with a solvent program of (I), a diethyl ether/hexane mixture (1:9, volume ratio) followed by 0.2 and 0.5 vol % isopropyl alcohol in (I). 13.1 mg of Chl-a and 7.6 mg of Chl-b were obtained from 24.7 mg of the partially purified chlorophyll using the above method.

• 公益社団法人 日本化学会の論文

著者

• IRIYAMA Keiji

  Research Institute for Polymers and Textiles

• SHIRAKI Masaru

  Research Institute for Polymers and Textiles

• OKADA Akira

  Research Institute for Polymers and Textiles

• Yoshiura Masahiko

  Research Institute for Polymers and Textiles

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• The crystal and molecular structures of esculetin 6-glucoside and 7-glucoside.
• Preparation of chlorophyll-a and chlorophyll-b by column chromatography with Sephasorb HP Ultrafine.

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