Insulin Release and Metabolism of Calcium, Adenine and Adenosine 3, 5-cyclic monophosphate
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概要
- 論文の詳細を見る
In order to assess further the mechanisms involved in insulin release, we prelabeled rat pancreatic islets of Langerhans by incubating either <SUP>45</SUP>Ca or [2-<SUP>3</SUP>H]adenine. When prelabeled islets were perfused with a glucose-free medium (the experiment with <SUP>45</SUP>Ca) and a medium containing 2.8mM glucose (the experiment with [2-<SUP>3</SUP>H] adenine) respectively, a constant rate of efflux of the radioactivity was established by 30min in each case. D-Glucose at 16.7mM concentration elicited a rapid efflux of <SUP>45</SUP>Ca and [2-<SUP>3</SUP>H] adenine derivatives ([<SUP>3</SUP>H]Ad) within 4 to 6 min after commencing the step-wise stimulation by glucose, concomitantly with insulin release. However, Lglucose and D-galactose little stimulated both <SUP>45</SUP>Ca and [<SUP>3</SUP>H] Ad release. Lanthanum chloride caused a burst peak of <SUP>45</SUP>Ca release in the absence of glucose. A rapid efflux of45Ca was caused by fi-D-glucose and D-glyceraldehyde to much lesser extent than by α-D-glucose.<BR>The slowly rising concentration of glucose at 0.1mm/min of gradient level failed to elicit any rapid efflux of <SUP>45</SUP>Ca or [<SUP>3</SUP>H] Ad, although insulin release occurred in accordance with an increase in glucose concentration. Even when the gradient of glucose concentration was raised to 0.7mM/min, glucose failed to stimulate an efflux of [<SUP>3</SUP>H] Ad but the subsequent stimulation by 16.7mM glucose caused a rapid efflux of [<SUP>3</SUP>H] Ad concomitantly with the release of insulin. No rapid efflux of <SUP>45</SUP>Ca was observed under a slow-rise glucose stimulation until the gradient level of the glucose concentration was raised to 6.7mM.<BR>Analysis of distribution of the radioactive adenine derivatives after incubation showed that the adenosine fraction had the highest radioactivity in the medium followed by the ATP, adenine and cAMP fraction in that order, and the ATP fraction had the highest radioactivity in the islet. The ratio of radioactivity in the cAMP fraction in the medium to the total count was the highest among all.<BR>On the basis of these results, it was suggested that the discharge of [<SUP>3</SUP>H] Ad and <SUP>45</SUP>Ca might occur with the alteration of the membrane permeability induced by a rapid change of the glucose concentration, and that their discharge might perhaps link to the glucoreceptor mechanism directly controlling insulin release.
- 社団法人 日本内分泌学会の論文
著者
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Kobayashi Kunio
Department Of Clinical Pathology And Clinical Laboratory Hyogo College Of Medicine
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Matsuoka Akira
Department Of Clinical Labolatory And Clinical Pathology Hyogo College Of Medicine
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Kagawa Shohei
Department Of Clinical Pathology And Clinical Laboratory Hyogo College Of Medicine
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Yoshida Keiko
Department Of Biology Faculty Of Science Niigata University
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Tsumura Yoko
Department Of Clinical Pathology And Clinical Laboratory Hyogo College Of Medicine
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