Insulin Release and Efflux of [<SUP>32</SUP>P] Phosphate from Islets of Rat Langerhans Treated with Iodoacetic Acid and the Anomers of D-glucose
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概要
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To clarify the insulin-releasing mechanism, we studied insulin release and the efflux of [<SUP>32</SUP>P] phosphate by glucose at 0.1mM/min of gradient level or at 16.7mM, and other metabolism in islets of rat Langerhans.<BR>When treated with 1mM iodoacetic acid (IAA) plus the anomers of D-glucose at2.8mM for6min at 37°C, islets elicited insulin at half the control rate under the step-wise stimulation by glucose and at the same rate as the control under the slow-rise stimulation by glucose. Using islets treated with IAA plus the α anomer at 16.7mM, the step-wise stimulation secreted insulin at half a rate of the control and the slow-rise stimulation at the rate lower than the control, which was not significantly different from the control rate. Treatment with IAA plus the β anomer at 16.7mM inhibited insulin release under both types of stimulations by glucose. The step-wise stimulation caused the same rapid efflux of [<SUP>32</SUP>P] phosphate from IAA treated islets as from the control islets, except for islets treated with IAA plus the β anomer at 16.7mM. The rate of glucose utilization in islets was inhibited by all IAA-treatments to the same extent, being merely half the control rate. Treatments with IAA plus the anomers at 16.7mM significantly reduced the formation of [<SUP>3</SUP>H]-cAMP and the activity of protein phosphokinase in islets, while in the presence of the anomers at 2.8mM IAA produced no significant effect. Neither IAA-treatments altered the uptake of <SUP>45</SUP>Ca and the ATP content in islets. The uptake of [<SUP>14</SUP>C] IAA was significantly enhanced by the presence of the β anomer at 16.7mM to two times the control level.<BR>On the basis of these results, we suggested that the B cell might contain both glucoreceptors and rate-sensors of glucose controlling insulin release and the former might be less sensitive to IAA as compared with the latter.
- 社団法人 日本内分泌学会の論文
著者
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Kobayashi Kunio
Department Of Clinical Pathology And Clinical Laboratory Hyogo College Of Medicine
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Matsuoka Akira
Department Of Clinical Labolatory And Clinical Pathology Hyogo College Of Medicine
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Kagawa Shohei
Department Of Clinical Pathology And Clinical Laboratory Hyogo College Of Medicine
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Yoshida Keiko
Department Of Biology Faculty Of Science Niigata University
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Tsumura Yoko
Department Of Clinical Pathology And Clinical Laboratory Hyogo College Of Medicine
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