亜鉛のけい光分析法における第4アンモニウム塩の効果

概要

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The fluorescence intensity of the chelate complex of Zn (II)-oxine-5-sulfonate is sensitized with the long-chain quaternary ammonium salt such as the zephiramine. By the use of this enhancement effect it is possible to increase the sensitivity of the fluorimetric determination of zinc. A fluorescent chelate complex of Zn(II)-oxine-5-sulfonate has its maximum emission at 526 nm with an excitation at 362 nm. As shown in figure 1, the addition of the quaternary ammonium salt produced a spectral shift of about 30 nm to longer wavelength in the excitation maximum and about threefold increase in the fluorescence intensity at the maximal.<BR>The fluorescence intensity and maximum wavelength of excitation of Zn(II)-oxine-5-sulfonate changed monotonously with decreasing the dielectric constant in dioxane-water mixtures. This suggests the large contribution of the “decreased dielectric constant” of micelle surface in water to the fluorescence enhancement. The addition of the zephiramine increased the fluorescence quantum efficiency of the complex from 0.022 to 0.042. The effect of diverse ions were examined with 2.0μg of Zn(II)/50 m<I>l</I>. The results are summarized in Table I. The ions, Mg(II), Sr(II), Ca(II), Ba(II), Cr(III), did not interfere. The ions, Cu(II), Co(II), Ni(II), completely quenched the fluorescence of the zinc chelate.
社団法人日本分析化学会の論文