魚の生体外排卵に関する生物学的研究-2-〔英文〕培養開始時間によるメダカの排卵率の違いについて

概要

論文の詳細を見る
This study was undertaken to determine the suitable starting hour of incubation and to accumulate some knowledge on time mapping during maturation and ovulation in Oryzias oocytes. At 10.00 a.m. the oocytes in the largest group were undergoing yolk formation. Therefore, the high percentages of ovulation in vitro were not obtained in both the cases using the CO2 incubator and usual method. The addition of hormones at 5.00 p.m. showed the effectiveness for inducing ovulation, as described in the previous work. The oocytes at this hour did not have the first type of micropinocytotic vesicles which were observed in the oocytes under yolk formation as seen under the electron microscope. At 10.00 p.m. and 12.00 p.m., some oocytes, incubated in the medium without any hormone, ovulated naturally. But each experimental group did not show higher ovulation rate than that at 5.00 p.m.. A complex and better conditions in tissue culture may be required by the oocytes as they approach the time of ovulation. The time mapping during the changing processes of maturation and ovulation is discussed.
日本水産學會の論文