魚体のカタラーゼについて-I : 魚体カタラーゼの測定法

概要

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Ten grams of fish liver were homogenized and made up to 100cc. with either 0.01M phosphate buffer solution (pH 6.8) or distilled water. It was kept in ice-box for 30 minutes and. centrifuged. The extracted solution was used for the determination with or without dilution, diluting liquid being the same as above. Details are given, in Tables 1-6. Sumners method answered the purpose well, but one minute interval of pipetting was found. sufficient for the determination, so that pipetting was done at 1, 2, 3, 4, 5 and 6 minutes instead. of Sumners original timing at 3, 6, 9 and 12 minutes. (Table 7 and Fig. 1). The present author proposed to define “catalase intensity” on the basis of the value k against. one gram of fresh tissue, instead of extractive dry matter. Catalase intensity (Catd. i.)=(kι→0)×n×d n……Times of extraction d……Times of dilution
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