Determination of biliary ionized Ca2+ concentration and its aspect in human gallbladder bile.

概要

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We analyzed total and ionized calcium in pathological human bile in association with biliary lipids. Ca2+ concentration was directly determined using conventional Ca2+ selective ion meter and a new type of sample container. The accuracy and precision of our ion selective electrode analysis were confirmed by similarity to ultrafiltrable Ca level cut-off molecular weight 1, 000. Total Ca levels ranged from 5.6 to 16.3mg/dl, and these levels were well correlated with biliary lipid concentrations. Unlike total Ca, Ca2+ concentration remained almost constant level (2.40±0.63mg/dl), regardless of the change in biliary lipid concentration. This findings was confirmed by the recovery test for addition of Ca2+ and dilution test adding saline solution to gallbladder bile. In conclusion, total Ca level was dependent on biliary lipid concentration, while Ca2+ level was independent and kept a constant value, probably owing to the regulating role of the mixed micelle in gallbladder bile.
社団法人日本肝臓学会の論文