肝特異F抗原:ヒト肝よりの分離・精製及び臨床的意義

概要

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Human liver-specific F antigen has been purified by the combination of centrifugation, block electrophoresis, Sephadex G-200 gel filtration, DEAE cellulose chromatography eluted with linear NaCl concentration gradient, and polyacrylamide gel electrophoresis. The purified F antigen had a molecular weight of approximately 80, 000 daltons. Specific antisera were prepared by immunizing guinea pigs with the purified F antigen. With the use of the antiserum, it was shown that human F antigen has a human specific determinant. Circulating F antigen in the serum of patients with various liver diseases was investigated. F antigen was most frequently found in the serum of patients with acute hepatitis, hepatocellular carcinoma and alcoholic hepatitis. Furthermore, F antigen was frequently found when SGOT and/or SGPT was over 100 IU/l.
社団法人日本肝臓学会の論文