Hypoxia differentially modulates the activity of pacemaker and smooth muscle cells in the guinea pig stomach antrum
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概要
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Effects of hypoxic solution (O2 tension, 161 ± 11 mmHg) on electrical responses of the membrane (slow waves), intracellular Ca2+-responses measured by Fura-2 fluorescence (Ca-transients) and isometric mechanical responses (phasic contraction) were observed in circular smooth muscles isolated from the guinea-pig stomach antrum. In normoxic solution (O2 tension, 362 ± 28 mmHg), muscle cells generated slow waves spontaneously, and switching to hypoxic solution caused an increase in frequency and decrease in duration of slow waves, with no significant change in the resting membrane potential. Hypoxia also reduced the amplitude and duration and increased the frequency of Ca-transients. The increase in frequency of slow waves by hypoxia was prevented by cyclopiazonic acid (CPA) but not by carbonyl cyanide m-chlorophenyl-hydrazone (CCCP), potassium cyanide (KCN) or low-Ca solution. The reduction by hypoxia of the duration of slow waves was prevented by CCCP or KCN but not by CPA or low-Ca solution. Hypoxia resulted in an increase in frequency and decrease in amplitude of phasic contractions, and the changes were prevented by CPA but not by CCCP. These results suggested that in antrum smooth muscle tissues, the increase in frequency of spontaneous activity by hypoxia is related to the enhanced function of the CPA-sensitive internal Ca-stores in pacemaker cells, while the inhibition in amplitude of phasic contractions by hypoxia may be mainly related to the decrease in Ca2+ release from the CPA-sensitive internal stores in smooth muscle cells. It is concluded that in hypoxic solution, the function of internal Ca2+ stores is enhanced in ICC-MY and is inhibited in smooth muscle cells in the guinea-pig stomach antrum.
著者
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Yamamoto Yoshimichi
Department Of Civil Engineering
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Yokoi Tsuyoshi
Department Of Biochemistry Gifu College Of Pharmacy
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Fukuta Hiroyasu
Department Of Physiology Nagoya City University Medical School
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Nakamura Eri
Department Of Cell Physiology Nagoya City University Medical School
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Suzuki Hikaru
Department Of Cardiology Tokyo Women's Medical University
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Yamamoto Yoshimichi
Department of Physiology, School of Nursing, Nagoya City University
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Iida Takao
Clinical Laboratory Medicine, Nagoya City University Hospital
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Tanaka Yasuo
Clinical Laboratory Medicine, Nagoya City University Hospital
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