Persistent Infection of Three Salmonid Cell Lines with Infectious Pancreatic Necrosis Virus(IPNV) (魚病に関する国際セミナ-)
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概要
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Persistent infections were established with infectious pancreatic necrosis virus (IPNV) in chinook salmon (CHSE-214), steelhead trout (STE-137) and in rainbow trout (RTG-2) cell lines. Viral persistence was characterized by the release of infectious virus, positive immunofluorescence, resistance to superinfection with homologous virus and susceptibility to challenges with heterologous viruses.<BR>The morphology and growth characteristics of persistently infected (PI) and uninfected cell lines was indistinguishable. PI CHSE-214 and STE-137 cell lines produce little or no interferon. Activity suggesting interferon was detected in culture fluids from PI RTG-2 cells.<BR>Temperature-sensitive virus was not detected in the culture fluids of PI CHSE-214 or STE-137 cell lines and the RTG-2 line has not been tested. Virus in the culture fluids of both PI STE-137 and RTG-2 cell lines demonstrated autointerference when inoculated onto uninfected control cells. The autointerfering component from PI STE-137 cells was removed from the culture fluid by ultracentrifugation and had a bouyant density of 1.29 g/cc in a cesium chloride gradient. Infectious virus also produced by PI STE-137 cells had a density of 1.33 g/cc. The production of defective interfering (DI) virus during viral persistence was further supported by electron microscopic examination of PI STE-137 cells. The production of DI virus by PI STE-137 cells as well as interferon in the PI RTG-2 cells were proposed as two mechanisms by which the cytocidal course of infection in these cell lines is prevented.
- 日本魚病学会の論文
著者
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FRYER J.L.
Department of Microbiology, Oregon State University
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Hedrick R.P.
Department of Medicine, School of Veterinary Medicine, University of California
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