DIFFERENTIAL EFFECTS OF D 600 ON RELEASE OF CATECHOLAMINES BY ACETYLCHOLINE, HISTAMINE, TYRAMINE AND BY CYCLIC AMP FROM CANINE ADRENAL MEDULLA

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The isolated canine adrenal glands were perfused retrogradely with Lockes solution, and the catecholamine contents of the effluents were measured by the trihydroxyindole-fluorimetric method. Stimulation of the glands by acetylcholine, histamine, tyramine and cyclic AMP caused an increase in release of catecholamines from the glands. Introduction of D 600 to the perfusion medium reduced release of catecholamines in response to acetylcholine, and this reduction was overcome by raising calcium ion concentrations of the perfusion medium. Similarly, D 600 reduced release of catecholamines in response to histamine. The release of catecholamines evoked by tyramine was also inhibited by D 600, although to a lesser degree than the release by acetylcholine. In contrast, D 600 was entirely ineffective on the cateeholamine release in response to cyclic AMP. D 600 had no effect on the spontaneous catecholaminc output. From these results it was concluded that release of catecholamines from adrenal chromaffin cells by acetylcholine and histamine, and by tyramine in part requires the entry of calcium ions across the cell membrane, whereas that by cyclic AMP does not.
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