Superprecipitation is a model for in vitro contraction superior to ATPase activity.
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概要
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We investigated which activity of myosin B (natural actomyosin), superprecipitation or ATPase, is better suited as a parameter for measuring in vitro contraction with emphasis on the response of myosin B to Sr<SUP>2+</SUP>. The Sr<SUP>2+</SUP> concentration giving half-maximum (K<SUB>Sr<SUP>2+</SUP></SUB>) binding of Sr<SUP>2+</SUP> to cardiac troponin was not different from that to skeletal troponin, whether the troponins were contained in the actomyosin system or in the isolated state. However, K<SUB>Sr<SUP>2+</SUP></SUB> for the ATPase activity of cardiac myosin B is slightly smaller than that of skeletal myosin B. This difference in K<SUB>Sr<SUP>2+</SUP></SUB> is more marked when superprecipitation was used as the indication for in vitro contraction. If the above results were compared with those of Kitazawa (1976) using glycerinated fibers of cardiac and skeletal muscle, the differences in K<SUB>Sr<SUP>2+</SUP></SUB> between cardiac and skeletal muscle increased in the order: Sr-binding to troponin<ATPase activity<superprecipitation<contraction of glycerinated fibers. The increase in the effect of pH on Ca<SUP>2+</SUP>-related properties is also in the order: Cabinding to troponin≤ATPase activity6lang;superprecipitation<contraction of glycerinated fibers. All the results coincide with one another that the results with superprecipitation are invariably more akin to those with glycerinated fibers than to those with the ATPase activity. It is concluded that superprecipitation is a better parameter for representing in vitro contraction than ATPase activity.
- 公益社団法人 日本薬理学会の論文
著者
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Kitaura Takashi
Laboratory Of Exercise Biochemistry College Of Liberal Arts Kanazawa University
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Kohama Kazuhiro
Department Of Molecular And Cellular Pharmacology Faculty Of Medicine Gunma University Graduate Scho
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EBASHI Setsuro
National Institute for Physiological Sciences
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HIRATA Masato
Department of Biochemistry, Faculty of Dentistry, Kyushu University
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SAIDA Kooichi
Department of Pharmacology, Hoshi College of Pharmacy
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KOHAMA Kazuhiro
Department of Pharmacology, Faculty of Medicine, University of Tokyo
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