Porcine Skeletal Muscle Dystrophin
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概要
- 論文の詳細を見る
With the aid of the antibodies raised against synthetic peptides corresponding to the sequences of human dystrophin, 440-489 and 3495-3544, respectively, dystrophin was isolated from porcine skeletal muscle as a homogeneous protein of about 400kDa. Main steps of preparation consisted of extraction with weakly alkaline Triton X-100 solution, dimethyl sulfoxide (DMSO) fractionation, gel filtration, cationic chromatography and preparative SDS gel electrophoresis.BrCN treatment of purified dystrophin gave rise to 40kDa and 10kDa fractions. Partial sequencing of the 40kDa fraction revealed that porcine dystrophin was more homologous with human dystrophin than was chicken dystrophin. The 10kDa fraction reacted strongly with the antibody against the peptide 3495-3544, but not to that against the peptide 440-489, and the sequences of its several parts examined were all identical with those of corresponding parts of human dystrophin, respectively.
著者
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Sugita Hideo
National Center Neurology And Psychiatry
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EBASHI Setsuro
National Institute for Physiological Sciences
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KUWAYAMA Hideto
Laboratory of Chemistry, Obihiro University of Agriculture and Veterinary Medicine
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SUGITA Hideo
National Institute of Neuroscience
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SUGIURA Masayo
National Institute for Physiological Sciences
関連論文
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