第一胃内発酵に関する研究 : II. in vitroにおけるガス生成能の測定

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An attempt was made to measure gas production in rumen contents by using a modified syringe, which also served as an incubation tool fitted to keep anaerobiosis of the rumen contents during the estimation. Its procedure included analysis for quantities of total gas, carbon dioxide and other gases. The rumen samples tested showed a linear pattern of gas production with the lapse of time for 6 hours of incubation. The gas produced by a given volume of a sample was thereby measured within 4-6 hours of incubation.Gas formation in the rumen was determined by this technique in two fistulated sheep. The capacity of carbon dioxide in the fluid phase of the rumen was changeable owing to pH fluctuation of the rumen followed by feed intake. After an intake of feed containing fresh Ladino clover and concentrates, gas production was suddenly increased in rumen samples. On the other hand, samples collected from a sheep fed orchard-grass hay and concentrates seemed to show a low production of gas. The proportions of carbon dioxide produced by amples collected from tow sheep were always from 70 to 76 per cent of the total gases.Some limitations for applying this technique to the study in vitro and standerd errors of measurements were discussed.
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