Small Interfering RNA (siRNA) against the Survivin Gene Increases Apoptosis in a Canine Melanoma Cell Line
スポンサーリンク
概要
- 論文の詳細を見る
The effect of down-regulation of survivin expression by small interfering RNA (siRNA) against the canine survivin gene on apoptosis was investigated by transfecting MCM-N1 (a canine malignant oral melanoma cell line) cells with siRNA using cationic liposomes. The siRNA against the canine survivin gene induced an increase in the rate of apoptotic cells and a decrease in the number of viable cells. We also measured sequence-specific down-regulation of survivin expression by reverse transcription-PCR and western blot analysis. The siRNA directed against survivin reduced both mRNA and protein expression in MCM-N1 cells. These findings suggest the importance of survivin in canine melanoma tumors for inducing apoptosis, and reinforce the possibility of using survivin as a putative therapeutic target in canine malignant melanoma tumor.
- 社団法人 日本獣医学会の論文
著者
-
MARUYAMA Haruhiko
Department of Pathobiology, College of Bioresource Sciences, Nihon University
-
KANO Rui
Department of Pathobiology, College of Bioresource Sciences, Nihon University
-
Kamata Hiroshi
Department Of Pathobiology Nihon University School Of Veterinary Medicine
-
Kano Rui
Department Of Pathobiology Nihon University School Of Veterinary Medicine
-
HASEGAWA Atsuhiko
Teikyo University Institute of Medical Mycology
-
Hasegawa Atsuhiko
Teikyo Univ. Inst. Medical Mycology Tokyo
-
MORIYAMA Miyuki
Department of Pathobiology, Nihon University School of Veterinary Medicine
-
HASEGAWA Astuhiko
Teikyo University Institute of Medical Mycology
-
Maruyama Haruhiko
Department Of Bioscience Department Of Cardiovascular Surgery
関連論文
- Establishment of Vertebral Heart Scale in the Growth Period of the Japanese Macaque (Macaca fuscata)
- Antifungal Activity of Itraconazole and Voriconazole against Clinical Isolates Obtained from Animals with Mycoses
- Molecular epidemiology of Arthroderma benhamiae, an emerging pathogen of dermatophytoses in Japan, by polymorphisms of the non-transcribed spacer region of the ribosomal DNA
- Fulminant Eosinophilic Myocarditis Associated With Visceral Larva Migrans Caused by Toxocara Canis Infection
- Small Interfering RNA (siRNA) against the Bcl-2 Gene Increases Apoptosis in a Canine Melanoma Cell Line
- 26S rDNA-Based Phylogenetic Investigation of Japanese Cattle-Associated Prototheca zopfii Isolates
- Reticulated Platelet Levels in Whole Blood and Platelet-Rich Plasma of Dogs with Various Platelet Counts Measured by Flow Cytometry(Clinical Pathology)
- Detection of Sporothrix schenckii chitin synthase 1 (CHS1) gene in biopsy specimens from human patients with sporotrichosis
- Candida albicans induced interleukin 8 production by human keratinocytes
- Bone Marrow Colony-Forming Unit Assay in Cats With Naturally Occurring Myelodysplastic Syndromes
- Cluster Analysis of Human and Animal Pathogenic Microsporum Species and Their Teleomorphic States, Arthroderma Species, Based on the DNA Sequences of Nuclear Ribosomal Internal Transcribed Spacer 1
- Detection of Microsporum canis in the Skin Scrapings and Hairs of Dogs with Dermatophytosis Based on Sequences of the Chitin Synthase 1 Gene
- Characterization of the Cyclophilin of Trichophyton mentagrophytes
- Chitin Synthase 2 Gene Sequence of Malassezia Species
- The First Isolation of Arthroderma benhamiae in Japan
- The First Isolation of Nocardia veterana from a Human Mycetoma
- First Isolation of Stephanoascus ciferrii from a Cat
- Enhanced UV♀1 tumor growth in CBF1 mice infected with Schistosoma mansoni due to modulation of Th1-like responses
- Intraepithelial infiltration of eosinophils and their contribution to the elimination of adult intestinal nematode, Strongyloides venezuelensis in mice
- Evaluation of the anthelmintic effects of artesunate against experimental Schistosoma mansoni infection in mice using different treatment protocols
- cDNA Sequencing and Expression of Rat Mast Cell Tryptase^1
- Research on calpain of Schistosoma japonicum as a vaccine candidate
- Adult worms of the rodent intestinal nematode, Strongyloides venezuelensis, successfully invatde chick intestinal mucosa
- Cloning and molecuar characterization of calpain, a calcium-activated neutral proteinase, from different strains of Schistoma japonicum
- Immune responses ageinst protozoal and nematodal infection in mice with underlyng Schistosoma mansoni infection
- Protective Effect of Vaccination with Toxoplasma Lysate Antigen and CpG as an Adjuvant against Toxoplasma gondii in Susceptible C57BL/6 Mice
- Expression of Inter-α-Trypsin Inhibitor Light Chain (Bikunin) in Human Pancreas
- Clinical Features and Outcomes of Eosinophilic Myocarditis Patients Treated with Prednisolone at a Single Institution Over a 27-Year Period
- INFLUENCES OF ALKALINE IONIZED WATER ON MILK ELECTROLYTE CONCENTRATIONS IN MATERNAL RATS
- Experimental Infection of Bovine Mammary Gland with Prototheca zopfii Genotype 1
- Small Interfering RNA (siRNA) against the Survivin Gene Increases Apoptosis in a Canine Melanoma Cell Line
- Case of tinea capitis caused by Trichophyton mentagrophytes (molecular type Arthroderma benhamiae) : Prevalence of a new zoonotic fungal infection in Japan
- Distinct Time Courses of Secondary Brain Damage in the Hippocampus Following Brain Concussion and Contusion in Rats
- A case report of pleural sparganosis
- Fine binding Specificities to Ascaris suum and Ascaris lumbricoides antigens of the sera from patients of probable visceral larva migrans due to Ascaris suum
- The First Case of Feline Prototheca wickerhamii Infection in Japan
- In Vitro Algaecide Effect of Disinfectants on Prototheca zopfii Genotypes 1 and 2
- Treatment of Canine Otitis Externa Using Video Otoscopy
- Fuchs' Heterochromic Iridocyclitis Associated with Ocular Toxocariasis
- Rapid identification of Prototheca zopfii by nested polymerase chain reaction based on the nuclear small subunit ribosomal DNA
- Zoonotic filariasis caused by Onchocerca dewittei japonica in a resident of Hiroshima Prefecture, Honshu, Japan
- Molecular typing and in-vitro activity of azoles against clinical isolates of Aspergillus fumigatus and A. niger in Japan
- Two Cases of Feline Orbital Aspergillosis due to Aspergillus udagawae and A. viridinutans
- In Vitro Activities of Antifungal Agents against Clinical Isolates of Dermatophytes from Animals
- A Case of Feline Sporotrichosis
- Genotyping of Malassezia pachydermatis Isolates from Canine Healthy Skin and Lesional Skin of Atopic Dermatitis in Japan, Korea and Taiwan
- Decreased Apoptotic Polymorphonuclear Leukocyte Rate in Dogs with Pyometra
- In Vitro Susceptibility of Malassezia pachydermatis Isolates from Canine Skin with Atopic Dermatitis to Ketoconazole and Itraconazole in East Asia