Small Interfering RNA (siRNA) against the Bcl-2 Gene Increases Apoptosis in a Canine Melanoma Cell Line
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概要
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The effects of down-regulation of Bcl-2 expression, by small interfering RNA (siRNA) against the canine Bcl-2 genes, on apoptosis were investigated by transfecting MCM-N1 (canine malignant oral melanoma cell line) cells with siRNA using cationic liposomes. The siRNA against the canine Bcl-2 genes increased the number of apoptotic cells. In addition, sequence-specific down-regulation of Bcl-2 expression was measured by RT-PCR and western blot analysis. The siRNA directed against these genes reduced both mRNA and protein expression in the MCM-N1 cells. Our study suggests the importance of Bcl-2 in canine melanoma tumors for inducing apoptosis and reinforces using Bcl-2 as a putative therapeutic target in canine malignant melanoma tumor.
- 社団法人 日本獣医学会の論文
著者
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MARUYAMA Haruhiko
Department of Pathobiology, College of Bioresource Sciences, Nihon University
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KANO Rui
Department of Pathobiology, College of Bioresource Sciences, Nihon University
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HASEGAWA Atsuhiko
Department of Pathobiology, College of Bioresource Sciences, Nihon University
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Kamata Hiroshi
Department Of Pathobiology Nihon University School Of Veterinary Medicine
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Kano Rui
Department Of Pathobiology Nihon University School Of Veterinary Medicine
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Watanabe Yuzuru
Department of Pathobiology, Nihon University School of Veterinary Medicine
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Watanabe Yuzuru
Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University
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Hasegawa Atsuhiko
Department Of Immunotherapeutics Tokyo Medical And Dental University Graduate School
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Maruyama Haruhiko
Department Of Bioscience Department Of Cardiovascular Surgery
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KAMATA Hiroshi
Department of Pathobiology, Nihon University School of Veterinary Medicine
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HASEGAWA Atsuhiko
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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