A Novel Sandwich Enzyme-Linked Immunosorbent Assay for Feline Insulin
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概要
- 論文の詳細を見る
A novel sandwich enzyme-linked immunosorbent assay (ELISA) was established to determine the serum insulin concentrations in domestic cats. By using a solid-phase mouse anti-bovine insulin monoclonal antibody and a peroxidase-conjugated guinea pig anti-rat insulin polyclonal antibody, feline serum insulin concentrations in the range of 0.1 to 3.6 ng/ml could be measured. The intraassay CV and interassay CV were less than 6% and less than 10%, respectively. The present insulin assay will strongly help studies on feline diabetes mellitus.
- 社団法人 日本獣医学会の論文
著者
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SHIBATA Haruki
Morinaga Institute of Biological Science, Inc.
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TAMAHARA Satoshi
Laboratory of Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, University o
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Matsuki Naoaki
Laboratory Of Veterinary Clinical Pathobiology Graduate School Of Agricultural And Life Sciences The
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Matsuki Naoaki
Department Of Veterinary Clinical Pathobiology Graduate School Of Agriculture And Life Sciences The
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Ono Kenichiro
Laboratory Of Veterinary Clinical Pathobiology
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NAKAYA Mariko
Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate
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KITO Yuko
Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate
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TOUHATA Yuki
Morinaga Institute of Biological Science Inc.
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ONO Kenichiro
Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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NAKAYA Mariko
Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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MATSUKI Naoaki
Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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SHIBATA Haruki
Morinaga Institute of Biological Science Inc.
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SHIBATA Haruki
Morinaga Institute of Biological Science
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