Parallel Reductions in Stomatin and Na,K-ATPase through the Exosomal Pathway during Reticulocyte Maturation in Dogs: Stomatin as a Genotypic and Phenotypic Marker of High K+ and Low K+ Red Cells
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概要
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Dogs can be divided into two genetic groups (a minor HK phenotype and a major LK phenotype) based on erythrocyte monovalent cation concentrations, which are controlled by the putative hk and lk allelic genes. HK dogs retain Na,K-ATPase in their erythrocytes due to the high activity of the enzyme in their precursor cells, whereas total loss of reticulocyte Na,K-ATPase occurs in LK dogs. Here, we report that the levels of the lipid raft-associated membrane protein stomatin decrease in parallel with those of Na,K-ATPase during reticulocyte maturation due to its extrusion in exosomes. The stomatin content of HK reticulocytes is higher than that of LK reticulocytes, and remains in the erythrocytes at levels compatible with that in human erythrocytes. However, it is almost absent from LK erythrocytes with the lk/lk genotype; similar to the deficiency seen in human red cells with overhydrated stomatocytosis. LK erythrocytes from hk/lk genotype dogs show reduced, but not negligible, levels of stomatin. These results indicate that the erythrocyte stomatin level is a suitable genotypic marker for the HK/LK red cell phenotype, and suggests a functional association between stomatin and Na,K-ATPase. The absence of morphological abnormalities in the erythrocytes of stomatin-deficient LK dogs also confirms that stomatin deficiency and stomatocytic shape change are independent from each other.
- 社団法人 日本獣医学会の論文
著者
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Otsuka Yayoi
Laboratory Of Internal Medicine Department Of Veterinary Clinical Science Graduate School Of Veterin
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Komatsu Tomohiko
Laboratory of Veterinary Internal Medicine, , Department of Veterinary Clinical Sciences, Graduate S
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Inaba Mutsumi
Laboratory of Molecular Medicine, Department of Veterinary Clinical Sciences, Graduate School of Vet
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Inaba Mutsumi
Laboratory Of Molecular Medicine Department Of Veterinary Clinical Sciences Graduate School Of Veter
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Sato Kota
Laboratory of Molecular Medicine, Graduate School of Veterinary Medicine, Hokkaido University
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ARASHIKI Nobuto
Laboratory of Molecular Medicine, Graduate School of Veterinary Medicine, Hokkaido University
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TANAKA Kohei
Laboratory of Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, University o
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TAMAHARA Satoshi
Laboratory of Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, University o
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ONO Ken-ichiro
Laboratory of Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, University o
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Arashiki Nobuto
Laboratory Of Molecular Medicine Department Of Veterinary Clinical Sciences School Of Veterinary Med
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Komatsu Tomohiko
Laboratory Of Veterinary Internal Medicine Department Of Veterinary Clinical Sciences School Of Vete
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