Identification of genes for two major sialoglycoproteins, glycophorin A and glycophorin C in canine red cell membranes
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Glycophorins are the major sialoglycoproteins in red blood cell membranes, possessing variousphysiological and pathological roles. We examined membrane glycoproteins in canine red cellsand cloned cDNAs for two major glycophorins, glycophorins A (GPA) and C (GPC) from bonemarrow cells. Periodic acid-Schiff staining and immunoblotting analyses showed that caninered cell membranes contained several glycoproteins immunoreactive to an anti-bovine GPC antibody,whereas the most abundant sialoglycoproteins, the candidates for GPA, did not reactwith an anti-human GPA antibody. The amino acid sequences of the extracellular domains ofGPA and GPC had no significant homology to those from other mammalian species, includinghumans, and had O-linked and/or N-linked glycosylation sites. On the other hand, the Cterminalcytoplasmic domain and/or the transmembrane helices of GPA and GPC were conservedamong species, indicating some functional significance of those regions in red cell membranesthat include dimerization of GPA in the membrane-spanning region, and association ofGPC with membrane skeletal proteins through binding with protein 4.1 and p55 in the cytoplasmicdomain. These findings provide insights for clinical studies to evaluate the involvementof GPA and GPC in the pathogenesis of red cell diseases.
- The Graduate School of Veterinary Medicine, Hokkaido Universityの論文
- 2008-03-07
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