細胞機能を利用したオリゴ糖鎖の合成
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概要
- 論文の詳細を見る
筆者らは動物細胞の機能を利用したオリゴ糖鎖の合成法を開発した。両親媒性の擬似糖脂質である糖鎖プライマーを培養液中に加えることで、1-2日後には糖鎖伸長生成物を培養液から回収できた。得られたオリゴ糖鎖の配列は細胞での糖鎖の生合成経路に依存しており、糖鎖プライマーと細胞の種類を変えることで、多種類のオリゴ糖鎖の合成が可能であった。糖鎖プライマーのアグリコン部分に官能基を導入することで、高分子化や基板への固定化を可能にした。糖鎖プライマー法はグライコミクスの新たな手法として期待される。
著者
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YAMAGATA Tatsuya
Laboratory of Glycoinformatics, Shenyang Pharmaceutical University
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Sato Toshinori
Department Of Biosciences And Informatics Faculty Of Science And Technology Keio University
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HATANAKA Kenichi
Center of Collaborative Research, University of Tokyo
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Sato Toshinori
Department Of Artificial Intelligence Kyushu Institute Of Technology
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Hashimoto Hironobu
National Institution for Academic Degrees and University Evaluation
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Hatanaka Kenichi
Center Of Collaborative Research University Of Tokyo
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Yamagata Tatsuya
Laboratory Of Glycoinformatics Shenyang Pharmaceutical University
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Sato Toshinori
Department of Applied Chemistry, Keio University
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Hatanaka Kenichi
Center for Collaborative Research (CCR) & Institute of Industrial Science (IIS), The University of Tokyo
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Yamagata Tatsuya
Laboratory of Tumor Biology and Glycobiology, Shenyang Pharmaceutical University
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