A novel method for analyzing formalin-fixed paraffin embedded (FFPE) tissue sections by mass spectrometry imaging
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概要
- 論文の詳細を見る
Significant advances have been made in the past decade in the field of mass spectrometry imaging (MS imaging). It is a relatively unestablished technique aimed at direct, high-sensitive and spatially exclusive detection of biological molecules from the surface of tissue sections, so that semi-quantitative distribution map of the analyte can be reconstituted from the mass spectra obtained. There is tremendous potential in its application especially in clinical field, such as biomarker discovery or pharmacokinetic study. However, vast majority of the work has been performed on frozen tissue sections, while it remains generally unpractical to produce frozen sections with clinically resected tumor samples. Here we report our novel sample preparation technique that enabled MS imaging from formalin-fixed paraffin embedded (FFPE) tissue section, including retrospective archive as old as 11 years. FFPE sections were first dewaxed with pre-warmed xylene, and exposed tissue surface was enzymatically digested in nanoliter scale droplets to retain analyte localization. As a result, we succeeded in obtaining MS images of peptide peaks derived from several proteins, identified by MS/MS analysis, using ovarian cancer FFPE sections. The qualities of mass spectra obtained by this method were not significantly different from those obtained from frozen sections. By this, we opened the door to retrospective study of past clinical cases in aim to discover molecular biomarker.(Communicated by Koichi TANAKA, M.J.A.)
- 日本学士院の論文
著者
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Aoki Daisuke
Department Of Aeronautics And Space Engineering Tohoku University
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Suzuki Atsushi
Department Of Animal Husbandry Faculty Of Agriculture Tohoku University
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Nakamura Yusuke
Laboratory For Genotyping Riken Snp Research Center C/o Riken Yokohama Institute
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AOKI Yutaka
Life Science Laboratory, Shimadzu Corporation
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TOYAMA Atsuhiko
Life Science Laboratory, Shimadzu Corporation
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SHIMADA Takashi
Life Science Laboratory, Shimadzu Corporation
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SUGITA Tetsuyoshi
Life Science Laboratory, Shimadzu Corporation
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AOKI Chikage
Life Science Laboratory, Shimadzu Corporation
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UMINO Yukari
Life Science Laboratory, Shimadzu Corporation
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DAIGO Yataro
Division of Advanced Clinical Proteomics, Institute of Medical Science, University of Tokyo
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SATO Taka-Aki
Life Science Laboratory, Shimadzu Corporation
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Shimada Takashi
Koichi Tanaka Lab. Of Advanced Sci. And Technol.
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