Integrity of Actin-Network Is Involved in Uridine 5'-Triphosphate Evoked Store-Operated Ca^<2+> Entry in Bovine Adrenocortical Fasciculata Cells
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概要
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Store-operated Ca2+ entry channels (SOCs) play an important role in the regulation of diverse non-excitable cell functions. However, the precise mechanism of SOCs activation is still controversial. Uridine 5-triphosphate (UTP) was shown to induce Ca2+ entry in a dihydropyridines-insensitive manner and accelerated steroidogenesis in bovine adrenocortical fasciculata cells (BAFCs) via the Gq/11 protein-coupled P2Y2 receptor. Therefore we investigated whether UTP is involved in SOCs activation and the mechanism of UTP-induced SOCs activation. Fura 2-loaded BAFCs were used for the measurement of intracellular concentration of Ca2+ ([Ca2+]i) mobilization. Extracellular UTP evoked Ca2+ release from intracellular stores followed by an increase in Ca2+ entry. The Ca2+ influx elicited by UTP was inhibited not by nifedipine, but by Zn2+, Cd2+, and Ni2+ (potency order: Zn2+ > Cd2+ >> Ni2+), and the effect of UTP was also attenuated by a phospholipase C inhibitor (U73122). These results indicate that UTP activates SOCs in BAFCs. The increase in [Ca2+]i by UTP was attenuated by ML-9, a myosin-light chain kinase inhibitor, and calmodulin inhibitors, W-7 and E6 berbamine, in a concentration-dependent manner. These reagents depolymerized actin filaments with rhodamine staining in BAFCs. Cytochalasin D also inhibited UTP-activated SOCs and depolymerized actin filaments. From these results, we proposed that calcium/calmodulin dependent myosin-light chain kinase is involved in the mobilization of actin filaments and the integrity of actin-network plays an important role in UTP-induced SOCs activation in BAFCs.
- 社団法人 日本薬理学会の論文
- 2003-01-01
著者
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KAWAMURA Masahiro
Department of Veterinary Internal Medicine, Faculty of Veterinary Medicine, Hokkaido University
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MASAKI Eiji
Department of Anesthesiology, Jikei University School of Medicine
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Masaki Eiji
Department Of Anesthesiology Jikei University School Of Medicine
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KONDO ICHIRO
Department of Anesthesiology, Jikei University School of Medicine
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TERASAKA Osamu
Division of Biology, Jikei University School of Medicine
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EBISAWA Takanori
Department of Pharmacology (I), Jikei University School of Medicine
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AHMED Ashram
Department of Anesthesiology, Ain Shams University
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KAGATA Miyuki
Department of Pharmacology (I), Jikei University School of Medicine
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Terasaka Osamu
Division Of Biology Jikei University School Of Medicine
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Terasaka Osamu
Division Of Biology Department Of Natural Science Jikei University School Of Medicine
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Kawamura M
Jikei Univ. School Of Medicine Tokyo Jpn
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Kawamura Masahiro
Department Of Pharmacology Department Of Internal Medicine Jikei University School Of Medicine
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Kondo Ichiro
Department Of Pharmacology (i) Jikei University School Of Medicine
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Ahmed Ashram
Department Of Anesthesiology Ain Shams University
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Kagata Miyuki
Department Of Pharmacology (i) Jikei University School Of Medicine
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Ebisawa Takanori
Department Of Pharmacology (i) Jikei University School Of Medicine
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Kondo Ichiro
Department Of Anesthesiology Jikei University School Of Medicine
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Kawamura Masahiro
Department Of Pharmacology (1) The Jikei University School Of Medicine
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