Application of Prohibitin Immunohistochemistry to Rat Livers Treated with Methapyrilene Hydrochloride
スポンサーリンク
概要
- 論文の詳細を見る
An immunohistochemical staining method for prohibitin, a 30-kDa protein located in the inner membrane of mitochondria, is used for detection of mitochondria on formalin-fixed paraffin-embedded sections. Methapyrilene hydrochloride (MP), which is known to increase mitochondria in periportal hepatocytes, was administered once by oral gavage to male rats at a dose of 100 mg/kg. Hypertrophy with eosinophilic granules was noted in the periportal hepatocytes treated with MP. Immunohistochemical staining with prohibitin antibody demonstrated a positive reaction to eosinophilic granules. Electron microscopy showed an increased number of mitochondria in the periportal hepatocytes treated with MP compared to the control. Although electron microscopy is a useful tool to detect ultrastructural changes, an immunohistochemical examination with anti-prohibitin antibody is a simple and easy method for detecting cytoplasmic mitochondria quantitatively.
- 日本毒性病理学会の論文
著者
-
Koujitani Takatoshi
Department Of Toxicology And Teratology Developmental Research Laboratories Dainippon Pharmaceutical
-
Seki Takaki
Safety Research Laboratories, Dainippon Sumitomo Pharma. Co., Ltd.
-
Michimae Yoshiko
Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd.
-
Toyosawa Kaoru
Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd.
-
Matsumoto Izumi
Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd.
-
Kouchi Mami
Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd.
-
Koujitani Takatoshi
Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd.
-
Inoue Tadashi
Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd.
-
Toyosawa K
大日本住友製薬安全性研究所
-
Mikami Shinichi
Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd.
-
Tanaka K
Department Of Gastrointestinal And Pediatric Surgery Division Of Reparative Medicine Institute Of Li
関連論文
- The First Case of Feline Infectious Peritonitis-like Pyogranuloma in a Ferret Infected by Coronavirus in Japan
- A Novel "Nihon" Rat Model of a Mendelian Dominantly Inherited Renal Cell Carcinoma
- Influences of biofluid sample collection and handling procedures on GC-MS based metabolomic studies(METHODS)
- Canine SPECT studies for cerebral amino acid transport by means of ^I-3-iodo-α-methyl-L-tyrosine and preliminary kinetic analysis
- Improvement of the embryonic stem cell test endpoint analysis by use of field potential detection
- Long-Term Treatment With Ranirestat (AS-3201), a Potent Aldose Reductase Inhibitor, Suppresses Diabetic Neuropathy and Cataract Formation in Rats
- Contrast-enhanced sonography of pancreatic carcinoma : correlations with pathological findings
- Improvement of the embryonic stem cell test endpoint analysis by use of field potential detection
- Giant mesenchymal hamartoma of the liver in an adult
- Porcelain gallbladder complicated with pancreas divisum
- Primary malignant peripheral nerve-sheath tumor of the common bile duct
- Long-term survival in a patient with hepatocellular carcinoma with resection of a metastatic lymph node after percutaneous ethanol injection therapy
- Risk factors of posthepatectomy liver failure after portal vein embolization
- Indications for curative resection of advanced gallbladder cancer with hepatoduodenal ligament invasion
- Adjuvant therapies using biliary stenting for malignant biliary obstruction
- Micrometastatic Colorectal Cancer Lesions in the Liver
- Tandem Repeats of Lactoferrin-Derived Anti-Hepatitis C Virus Peptide Enhance Antiviral Activity in Cultured Human Hepatocytes
- Mitotic Checkpoint Protein hsMAD2 as a Marker Predicting Liver Metastasis of Human Gastric Cancers
- Application of Prohibitin Immunohistochemistry to Rat Livers Treated with Methapyrilene Hydrochloride
- Mucinous Adenocarcinoma of the Jejunum in a F344 Rat
- Assessment of T cell dependent antibody response, hematology, pathology and immunophenotyping in the identical rat on immunotoxicology studies : Immunotoxicology study with Cyclophosphamide(Proceedings of the 32nd Annual Meeting)
- Expression of Vascular Endothelial Growth Factor (VEGF) Family Members in Breast Cancer
- Relationship between liver weight change and gene expression of hepatic cytochrome P450 isoforms in clobazam-treated rats(Proceedings of the 32nd Annual Meeting)
- Usefulness of field potential as a marker of embryonic stem cell-derived cardiomyocytes, and endpoint analysis of embryonic stem cell test
- Roles of Extracellular Signal-regulated Kinase 1/2 and p38 Mitogen-activated Protein Kinase in the Signal Transduction of Basic Fibroblast Growth Factor in Endothelial Cells during Angiogenesis
- Clotrimazole, an Imidazole Antimycotic, Is a Potent Inhibitor of Angiogenesis
- Simultaneous Bindings of uPA and Latent TGF-β for the Activation of Latent TGF-β in Homotypic Smooth Muscle Cells
- Intravital imaging of DSS-induced cecal mucosal damage in GFP-transgenic mice using two-photon microscopy
- Perioperative Host-Tumor Inflammatory Interactions : A Potential Trigger for Disease Recurrence Following a Curative Resection for Colorectal Cancer
- Ultrasonically Activated Shears in Gastrectomy for Large Gastric Cancers
- Operative Morbidity Associated with Groin Dissections
- Spontaneous Fibrosarcoma with Pleomorphic Appearance in an Aged Brown Norway Rat
- Liver transplantation for primary sclerosing cholangitis
- Minimum sizes for remnant and transplanted livers in rats
- Application of Prohibitin Immunohistochemistry to Rat Livers Treated with Methapyrilene Hydrochloride
- DPEP1, expressed in the early stages of colon carcinogenesis, affects cancer cell invasiveness
- Multiple Solitary Leiomyomas in the Esophagus : Report of a Case
- Immunohistochemical and Ultrastructural Analyses of Cytoplasmic Blood Plasma Inclusions of Rat Hepatocytes
- Prognostic significance of glucose transporter-1 (GLUT1) gene expression in rectal cancer after preoperative chemoradiotherapy
- Cystic Cholangioma in the Thoracic Cavity of a Rat
- The First Case of Feline Infectious Peritonitis-like Pyogranuloma in a Ferret Infected by Coronavirus in Japan
- High TrkB expression levels are associated with poor prognosis and EMT induction in colorectal cancer cells
- Spontaneous Fibrosarcoma with Pleomorphic Appearance in an Aged Brown Norway Rat
- Immunohistochemical and Ultrastructural Analyses of Cytoplasmic Blood Plasma Inclusions of Rat Hepatocytes
- Immunohistochemical and Ultrastructural Analyses of Cytoplasmic Blood Plasma Inclusions of Rat Hepatocytes