Effect of Extracellular Matrix on Gene Expression and mRNA Stability in Primary Rat Hepatocytes
スポンサーリンク
概要
- 論文の詳細を見る
Liver regeneration is accompanied by a series of profound changes in hepatocyte gene expression. Similar changes in gene expression occur when hepatocytes are placed in primary culture. In the present study, we wished to determine the in vitro patterns of gene expression for several different functional classes of hepatocyte genes, and whether the changes in gene expression depended on the extracellular matrix. We therefore examined the expression of several genes which are known to change during hepatocyte proliferation in vivo or which are known to participate in different aspects of differentiated hepatocyte function. Hepatocytes were plated on collagen Type I, a matrix which supports cellular proliferation, or on the basement membrane matrix Matrigel, which supports a non-proliferating, differentiated hepatocyte phenotype. Total cellular RNA was collected after various times in culture, and cellular mRNA levels were determined by northern blot analysis for the following: transcription factors liver regeneration factor-1 (LRF-1) and CCAAT/enhancer binding protein delta (CEBPδ), the microsomal enzyme cytochrome CYP2A3 (rat orthologue of mouse CYP2A5) and the cell surface heparan sulfate proteoglycan, syndecan-2 (syn-2). Marked and rapid increases in LRF-1 and CEBPδ expression were observed on both matrix types. However, the expression of LRF-1 decreased rapidly on both matrices at 24 h, whereas CEBPδ expression was more delayed and returned to baseline values after 48 h. Messenger RNA for CYP2A3 decreased over 24 h on both matrices, but returned to 46% of control after 48 h on Matrigel which was significantly higher than hepatocytes plated on collagen. Finally, the expression of syndecan-2 was markedly decreased when hepatocytes were cultured on collagen Type I, but remained higher for hepatocytes grown on Matrigel at 48 h. For syndecan-2 it was shown that the half-life of syndecan mRNA was 1.5 fold longer on Matrigel than on collagen for the 3.4 kb mRNA, but no differences were found for the 2.2 and 1.1 kb messenger RNAs. These studies suggest that many changes in gene expression which occur in vivo during liver regeneration also occur in vitro during the transition to culture, and that some of these changes are influenced by the matrix upon which the cells are grown. Furthermore, these studies demonstrate that matrix-dependent changes in mRNA stability contribute, at least in part, to the observed changes in gene expression.
- 日本毒性病理学会の論文
- 1998-12-01
著者
-
YAMATE JYOJI
Department of Veterinary Pathology, Graduate School of Agricultural Lifesciences
-
TSUKAMOTO Yasuhiro
Department of Anatomy and Developmental Neurobiology, Kobe University Graduate School of Medicine
-
Yamate Jyoji
Department Of Veterinary Pathology Graduate School Of Agriculture And Biological Science Osaka Prefe
-
BENN Sally
Department of Biomedical Sciences, University of Guelph
-
ALLAN Alison
Department of Biomedical Sciences, University of Guelph
-
LAITHWAITE James
Department of Biomedical Sciences, University of Guelph
-
KIRBY Gordon
Department of Biomedical Sciences, University of Guelph
-
LAMARRE Jonathan
Department of Biomedical Sciences, University of Guelph
-
Benn Sally
Department Of Biomedical Sciences University Of Guelph
-
Kirby Gordon
Department Of Biomedical Sciences University Of Guelph
-
Allan Alison
Department Of Biomedical Sciences University Of Guelph
-
Lamarre Jonathan
Department Of Biomedical Sciences University Of Guelph
-
Laithwaite James
Department Of Biomedical Sciences University Of Guelph
-
Tsukamoto Yasuhiro
Department Of Anatomy And Developmental Neurobiology Kobe University Graduate School Of Medicine
-
Tsukamoto Yasuhiro
Department Of Veterinary Pathology Osaka Prefecture University
-
YAMATE Jyoji
Department of Integrated Structural Biosciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
関連論文
- Preferential Induction of Mammary Tumors in p53 Hemizygous BALB/c Mice by Fractionated Irradiation of a Sub-lethal Dose of X-rays
- Age-dependent Decrease in Incidence of Rat Sperm Granulomas Induced by L-Cysteine
- Macrophage Populations, Myofibroblastic Cells, and Extracellular Matrix Accumulation in Chronically-Developing Rat Liver Cirrhosis Induced by Repeated Injection of Thioacetamide
- Phenotypic modulation in cisplatin-resistant cloned cells derived from transplantable rat malignant fibrous histiocytoma
- MORPHOLOGY OF ARTERIOSCLEROSIS IN AGED RATS LOADED WITH VITAMIN D_2 AND CHOLESTEROL
- Retrograde labeling of mouse spinal descending tracts by a recombinant adenovirus
- A Susceptibility Locus for Radiation Lymphomagenesis on Mouse Chromosome 16
- Effects of Gadolinium Chloride (GdCl_3) on Macrophage Populations in Acute Model Induced in Rats by Thioacetamide(Proceedings of the 32nd Annual Meeting)
- Rapid Insulin-like Growth Factor-1-induced Changes in Granulosa Cell Thrombospondin-1 Expression In Vitro
- Nephrotoxicity of a novel antineoplastic platinum complex, nedaplatin : a comparative study with cisplatin in rats(Proceedings of the 32nd Annual Meeting)
- Metabolic Fingerprinting in Toxicological Assessment Using FT-ICR MS
- Amelioration of Nedaplatin-Induced Nephrotoxicity by Continuous Infusion in Rats
- Expression of Insulin-Like Growth Factor (IGF) Family Members in Porcine Pregnancy
- Effect of Extracellular Matrix on Gene Expression and mRNA Stability in Primary Rat Hepatocytes
- Age-related changes in growth hormone-immunoreactive cells in the anterior pituitary gland of Jcl : Wistar-TgN (ARGHGEN) 1Nts rats (Mini rats)
- Successful Management with CHOP for Pulmonary Lymphomatoid Granulomatosis in a Dog
- Comparative Nephrotoxicity of Cisplatin and Nedaplatin:Mechanisms and Histopathological Characteristics
- Effect of azuki bean seed coat on renal interstitial fibrosis of rats induced by cisplatin (KIDNEYS AND URINARY EXCRETION SYSTEM) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
- Rapid Insulin-like Growth Factor-1-induced Changes in Granulosa Cell Thrombospondin-1 Expression In Vitro
- Extension of Density Functional Theory to Nuclear Orbital plus Molecular Orbital Theory : Self-Consistent Field Calculations with the Colle-Salvetti Electron-Nucleus Correlation Functional
- Expression of Insulin-like Growth Factor (IGF) Family Members in Porcine Pregnancy
- Predictive genomic biomarkers for drug-induced nephrotoxicity in mice
- Metabolic Fingerprinting in Toxicological Assessment Using FT-ICR MS
- Malignant Melanoma with Cartilaginous Formation in a Dog
- Cisplatin-Induced Rat Renal Interstitial Fibrosis ; A Possible Pathogenesis Based on the Data
- Comparative Nephrotoxicity of Cisplatin and Nedaplatin : Mechanisms and Histopathological Characteristics
- Epithelial Cell Kinetics in the Inflammatory Process of Chicken Trachea Infected with Infectious Bronchitis Virus.