サルモネラ菌の分岐鎖アミノ酸LIV-2輸送系の膜たんぱく成分を支配するbrnQ遺伝子のクロ-ニング及びその塩基配列〔英文〕
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概要
- 論文の詳細を見る
The genetically difined gleR-brnQ region responsible for the branched-chain amino acid transport in Salmonella typhimurium was mapped in the 3.3-kilobase SalI-PstI segment of plasmid pOH56 by complementation analysis. By subcloning and genetic recombination analysis, the gleR and brnQ3 mutational sites were localized within the 0.85-kilobase SalI-EcoRV segment, and brnQ4 within the 0.8-kilobase EcoRV-HindIII segment. The nucleotide sequence of the brnQ gene and its flanking regions was determined. The brnQ gene is encoded by the sequence starting 24 base pairs upstream from the EcoRV site. Transcription of the brnQ gene starts at three sites separated by 171, 173 and 174 nucleotides, respectively, from the initiation colon. The promoter sequences can be seen in the immediate upstream region of the transcription initiation sites. There is a long silent region between the transcription initiation sites and a potential Shine-Dalgarno nucleotide sequence. The coding sequence of the brnQ gene, which is 1317 base pairs long, specifies a very hydrophobic protein of 439 amino acid residues.
- 日本遺伝学会の論文
著者
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Ohnishi Kuniharu
Department Of Microbiology Department Of Pharmaceutical Sciences.hokuriku University
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Matsubara Keiko
Department Of Cardiology Rakusai Newtown Hospital
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Hasegawa Akira
Department Of Applied Bioorganic Chemistry Gifu University
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大西 邦治
Department of Microbiology, School of Pharmacy, Hokuriku University
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Date Takayasu
Department Of Biochemistry Kanazawa Medical University
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KIRITANI KAZUYOSHI
Department of Microbiology, School of Pharmacy, Hokuriku University
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OKADA Toshihiko
Department of Biochemistry and Kanazawa Medical Universit
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HASEGAWA Akira
Department of Microbiology, School of Pharmacy, Hokuriku University
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KIRITANI KAZUYOSHI
Department of Bacteriology, Medical School, Kanazawa University
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MATSUBARA Keiko
Department of Microbiology, School of Pharmacy, Hokuriku University
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長谷川 晃
Department of Microbiology, School of Pharmacy, Hokuriku University
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松原 京子
Department of Microbiology, School of Pharmacy, Hokuriku University
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