DNAアレイによる着床過程に関与する新規遺伝子群の探索

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概要

• 論文の詳細を見る

• Conceptus implantation to the mothers uterus is a complex series of events involving coordinated expression of numerous genes at both the embryonic and the uterine sides. Since there are no suitable in vivo or in vitro experimental models, sequential changes occurring during the peri-implantation periods have not been well characterized. Using GeneChip technology and a recently introduced murine in vitro model of implantation, the expression of embryonic genes was examined before and after attachment to the uterine stromal cells. Instead of RNA or mRNA, amplified cRNA was subjected to the GeneChip analysis because amounts of mRNA in each blastocyst were minimal. Among 6,500 gene transcripts examined, changes in mRNA levels for 802 genes were identified. Of these detections, transcripts previously unsuspected were changes in a group of tumor suppressor and stress-induced genes, whose transcripts increased as embryos attached to the membrane. Validity of the data was evaluated using reverse transcription-polymerase chain reaction and in situ hybridization analyses, both of which confirmed developmental changes in selected gene expressions during pre- and post-attachment periods. The present data suggest that GeneChip technology would be very useful for finding genes previously unsuspected, and this method should be used as an initial step, particularly as a screening tool, toward the dissection of complex mechanisms such as the processes of implantation.

• 日本繁殖生物学会の論文

著者

• 高橋 祐司

  東京大学大学院農学生命科学研究科動物育種繁殖学教室

• 今井 美沙

  東京大学大学院農学生命科学研究科動物育種繁殖学教室

• 秦 俊文

  Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo

• 高橋 祐司

  Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo

• 今井 美沙

  Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo

• YAMAMOTO Shinya

  Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo

• TAKAKURA Kenji

  Department of Obstetrics and Gynecology, Shiga University of Medical Sciences

• NODA Youichi

  Department of Obstetrics and Gynecology, Shiga University of Medical Sciences

• IMAKAWA Kazuhiko

  Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo

• Takahashi Yuji

  Laboratory of Environmental Molecular Physiology, School of Life Sciences, Tokyo University of Pharm

• QIN Junwen

  Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo

• IMAI Misa

  Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo

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