Production of Calcium Maintenance Factor Stanniocalcin-1 (STC1) by the Equine Endometrium During the Early Pregnant Period

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概要

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• A factor responsible for progression to pregnancy establishment in the mare has not been definitively characterized. To identify factors possibly involved in the establishment of equine pregnancy, the endometrium was collected from day 13 (day 0=day of ovulation) cyclic and day 13, 19 and 25 pregnant animals. From initial subtractive hybridization studies, a calcium regulating factor, Stanniocalcin-1 (STC1) mRNA, was found as a candidate molecule expressed uniquely in the pregnant endometrium. Endometrial expression of STC1 mRNA was noted on day 19 and was markedly increased in the day 25 gravid endometrium. STC1 protein was found in the extracts of day 25 gravid endometrium and immunochemically localized in the uterine glands. In addition, STC1 protein was detected in uterine flushing media collected from day 25 pregnant mares. High concentrations of estradiol-17β (E 2) were detected in day 25 conceptuses. E2 levels were much higher in the gravid endometrium than in other regions, whereas progesterone levels did not differ among the samples from different endometrial regions. Expression of STC1 mRNA, however, was not significantly upregulated in cultured endometrial explants treated with various concentrations of E2 (0.01-100 ng/ml) with or without 10 ng/ml progesterone. These results indicate that an increase in STC1 expression appears to coincide with capsule disappearance in the conceptus, and suggest that STC1 from the uterine glands likely plays a role in conceptus development during the pregnancy establishment period in the mare.

著者

• IMAKAWA Kazuhiko

  Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo

• NAGAOKA Kentaro

  Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo

• NAMBO Yasuo

  Hidaka Training Research Center

• MATSUI Motozumi

  Laboratory of Theriogenology, Department of Veterinary Clinical Science, Graduate School of Veterina

• KIKUCHI Masato

  Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo

• NAKANO Yasuko

  Laboratory of Animal Breeding, Faculty of Agriculture, The University of Tokyo

• HANEDA Shingo

  Laboratory of Theriogenology, Obihiro University of Agriculture and Veterinary Medicine

• MIYAKE Yoichi

  Laboratory of Theriogenology, Obihiro University of Agriculture and Veterinary Medicine

• MACLEOD James

  Gluck Equine Center, Department of Veterinary Science, University of Kentucky

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