The Antiandrogenic Activity of the Fungicide N-(3, 5-Dichlorophenyl) Succinimide in In vivo and In vitro Assays
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概要
- 論文の詳細を見る
Antiandrogens can cause reproductive disorders in humans and wild animals. In the present study, we tested whether the fungicide N-(3, 5-dichlorophenyl) succinimide (NDPS) acts as an antiandrogen using in vitro and in vivo assays. A transient transfection system based on luciferase activity was utilized for in vitro analysis of the antiandrogeic activity of NDPS. Hershberger assay was used to analyze the antiandrogenic activity of NDPS in rats. The expressions of the androgen-responsive genes testosterone-repressed prostatic message-2 (TRPM-2) and prostate specific binding protein polypeptide C3 (PBP C3) in the rat ventral prostate were measured using real-time PCR. Our results indicated that NDPS can block 5-dehydrotestosterone (DHT)-induced androgen receptor (AR) activity in transiently transfected HepG2 cells (-5 Log M). In the Hershberger assay, the weights of the seminal vesicles and levator ani/bulbocavernosus muscles were significantly decreased (P<0.05) in all NDPS groups, and the weights of the ventral prostate, dorsolateral prostate, and Cowpers glands were significantly decreased (P<0.05) in the 100 and 200 mg/kg NDPS groups. NDPS only decreased (P<0.05) the expression of PBP C3 and had no effect on the level of TRPM-2 (P>0.05). In conclusion, NDPS is a moderate antiandrogen that elicits antiandrogenic effects at least partly by antagonizing AR and increasing the expression of PBP C3.
- 日本繁殖生物学会の論文
著者
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Zhu Xinqiang
Department Of Environmental Health School Of Medicine Fukui Medical University
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Yang Jun
Department Of Bioscience And Biotechnology Institute Of Bioscience Sejong University
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Zheng Yifan
Department Of Chemistry Zhejiang University
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Zhang Jun
Department Of Bioengineering And Robotics Graduate School Of Engineering Tohoku University
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ZHANG GuoJun
Health Inspection Office of Hangzhou Health Bureau
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ZHU HuiJuan
Department of Toxicology, School of Medicine, Zhejiang University
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YAO GengDong
Department of Occupational and Environmental Health, School of Medicine, Zhejiang University
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Yang Jun
Department Of Biology Sichuan Teachers College
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Zhang Jun
Department Of Anesthesiology Huashan Hospital Fudan University
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ZHENG YiFan
Department of Toxicology, School of Medicine, Zhejiang University
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YANG Jun
Department of Toxicology, School of Medicine, Zhejiang University
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