Protective Effects of Quercetin and Its Metabolites on H_2O_2-Induced Chromosomal Damage to WIL2-NS Cells
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概要
- 論文の詳細を見る
We investigated chromosomal damage caused by a typical flavonoid, quercetin, and its two conjugates, quercetin-3-O-sulfate and isorhamnetin, and their protective effects against chromosomal damage induced by H2O2. The chromosomal damage was detected by the cytokinesis-block micronucleus (CBMN) assay using a lymphoblastoid cell line, WIL2-NS. We found that quercetin itself induced chromosomal damage at 10 μM, but quercetin-3-O-sulfate and isorhamnetin did not induce damage up to 30 μM. In the medium used for the CBMN assay, quercetin (at 100 μM) generated a high concentration of H2O2, but the two conjugates did not at the same concentration. On the other hand, pretreatment with quercetin (at 1 μM), quercetin-3-O-sulfate (at 10 μM), and isorhamnetin (at 5 μM) prevented H2O2-induced chromosomal damage to WIL2-NS cells. These findings suggest that the induction and prevention of H2O2-induced chromosomal damage are different between quercetin and its metabolites.
- 社団法人 日本農芸化学会の論文
- 2004-02-23
著者
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Umegaki Keizo
National Institute Of Health And Nutrition
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SAITO AKIKO
Hokkaido Institute o Pharmaceutical Sciences
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Saito Akiko
Hokkaido Institute Of Public Health
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SUNAGAWA Hiroyuki
Hokkaido Institute of Public Health
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SUGISAWA Ayako
National Institute of Health and Nutrition
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Umegaki Keizo
National Inst. Of Health And Nutrition
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SUNAGAWA Hiroyuki
Hokkaido Institute of Public Healt
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