A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm
スポンサーリンク
概要
- 論文の詳細を見る
We constructed a new plasmid vector for the production of a modified silk fibroin heavy chain protein (H-chain) in the transgenic silkworm. The plasmid (pHC-null) contained the promoter and the 3′ region of a gene encoding the H-chain and the coding regions for the N-terminal domain and the C-terminal domain of the H-chain. For the model protein, we cloned a foreign gene that encoded EGFP between the N-terminal domain and the C-terminal domain in pHC-null and generated transgenic silkworms that produced a modified H-chain, HC-EGFP. Transgenic silkworms produced HC-EGFP in the posterior part of silk gland cells, secreted it into the lumen of the gland, and produced a cocoon with HC-EGFP as part of the fibroin proteins. N-terminal sequencing of HC-EGFP localized the signal sequence cleavage site to between positions A(21) and N(22). These results indicate that our new plasmid successfully produced the modified H-chain in a transgenic silkworm.
- 社団法人 日本農芸化学会の論文
著者
-
Tamada Yasushi
National Institute Of Agrobiological Sciences
-
Kojima Katsura
National Institute Of Agrobiological Sciences
-
Tamura Toshiki
National Institute of Agrobiological Sciences
-
Kojima Katsura
National Inst. Agrobiological Sci. Ibaraki Jpn
-
Kobayashi Isao
National Food Research Institute
-
KUWANA Yoshihiko
National Institute of Agrobiological Sciences
-
Sezutsu Hideki
National Institute of Agrobiological Sciences
-
UCHINO Keiro
National Institute of Agrobiological Sciences
関連論文
- Development of Microchannel Emulsification Technology for Monodispersed Soybean and Olive Oil-in-Water Emulsions
- Cloning and characterization of a β-N-acetylglucosaminidase (BmFDL) from silkworm Bombyx mori(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Preparation Characteristics of Lipid Microspheres Using Microchannel Emulsification and Solvent Evaporation Methods
- Design and Fabrication of Biosensor Device by Use of Receptor Proteins
- 1P-327 高感度バイオセンサのための組換え昆虫匂いレセプタの発現(バイオエンジニアリング,第46回日本生物物理学会年会)
- Identification of Four Major Hornet Silk Genes with a Complex of Alanine-Rich and Serine-Rich Sequences in Vespa simillima xanthoptera Cameron
- A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm
- 1P514 Potential Measurement of Lipid Bilayer Membrane by Microfluidic FET Device(25. New methods and tools (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Evaluating Promoter Sequences for Trapping an Enhancer Activity in the Silkworm Bombyx mori
- FE-P13 Premix microchannel emulsification using uniform droplets : Effects of channel dimension and operating condition(Section IX Food Engineering)
- FE-O6 Controllable production of nonspherical lipid droplets and microparticles using microchannel array devices(Section IX Food Engineering)
- Breakup of Oil-in-Water Emulsion Droplets in Microchannel Array Devices at Low Applied Pressures
- CFD Analysis of Generation of Soybean Oil-in-Water Emulsion Droplets Using Rectangular Straight-Through Microchannels
- CFD Study of the Effect of a Fluid Flow in a Channel on Generation of Oil-in-Water Emulsion Droplets in Straight-Through Microchannel Emulsification
- An Improved DNA Injection Method for Silkworm Eggs Drastically Increases the Efficiency of Producing Transgenic Silkworms
- FE-P20 Preparation of lipid microcapsules that entrap water-soluble materials using water-in-oil-in-water multiple emulsions via solvent evaporation(Section IX Food Engineering)
- FE-O2 Simulation of motion of gastric fluid induced by peristalsis using CFD(Section IX Food Engineering)
- In vitro Study of Initial Cell Attachment to the Surfaces Coated with Conjugates Consisting of Silk Fibroin and Chitooligosaccharides
- Conservation of fibroin gene promoter function between the domesticated silkworm Bombyx mori and the wild silkmoth Antheraea yamamai
- Development of a New piggyBac Vector for Generating Transgenic Silkworms using the Kynurenine 3-mono Oxygenase Gene
- Production and Characterization of Monodispersed Oil-in-Water Microspheres Using Microchannels
- IDENTIFICATION OF W-3 GENE WHOSE MUTATIONS MAKE SILKWORM LARVAL SKIN TRANSLUCENT BECAUSE OF URIC ACID TRANSPORTATIONI DEFICIENCY(Genetics,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm
- Identification of Four Major Hornet Silk Genes with a Complex of Alanine-Rich and Serine-Rich Sequences in Vespa simillima xanthoptera Cameron
- Molecular Cloning and Expression of Two Novel β-N-Acetylglucosaminidases from Silkworm Bombyx mori
- Attempt at Trangenesis of the Silkworm (Bombyx mori L.) by Egg-Injection of Foreign DNA
- Suppression of Fibroblast Cell Growth on Surfaces Coated with Conjugates Consisting of Silk Fibroin and Chitooligosaccharides
- Rescue of the Aojuku white-age translucent (w-3^) Bombyx mori mutant by transgenic expression of the wild-type Bmwh3 gene
- Evaluation of Calcium Phosphate-Coated Silk Fabric Produced by Sol-Gel Processing as a Wound Cover Material
- Extensive Sequence Rearrangements and Length Polymorphism in Fibroin Genes in the Wild Silkmoth, Antheraea yamamai (Lepidoptera, Saturniidae)
- Uniform size of leucine-rich repeats in a wild silk moth Saturnia japonica (Lepidoptera Saturniidae) fibroin
- Nucleotide sequence variation in mitochondrial COI gene among 147 silkworm (Bombyx mori) strains from Japanese, Chinese, European and moltinism classes
- Silk Genes in Wild Silk Moths
- Transient expression of chimeric CAT genes injected into early embryos of the domesticated silk worm Bombyx mori.