Attempt at Trangenesis of the Silkworm (Bombyx mori L.) by Egg-Injection of Foreign DNA
スポンサーリンク
概要
- 論文の詳細を見る
We analysed the feasibility of transforming the silkworm germ line cells by random insertion of foreign DNA. Cloned DNA, either supercoiled or linear, introduced into eggs at early stages of development was submitted to intense degradation after injection, but a stable pool of intact sequences persisted throughout embryogenesis in both embryonic and non-enbryonic tissues. Screenings of germ line transformants were carried out by tracking injected foreign sequences in genomic DNA of individuals at the G0 and G1 generations. One case of germ line transmission of foreign DNA was observed among more than 500 broods analysed. Distribution of the transgene among G1 individuals agrees with Mendelian inheritance from the G0 to the G1 generation. The integrated sequence was modified compared to the originally injected one. This study shows that foreign DNA can integrated into the chromosomes of the silkworm germ lineage but than improving the method of making transgenics requires the development of gene vector systems.
- 日本応用動物昆虫学会の論文
- 1996-11-25
著者
-
Tamura Toshiki
National Institute of Agrobiological Sciences
-
Kanda Toshio
National Institute Of Sincultural And Entomological Science
-
Yukuhiro Kenji
National Institute of Agrobiological Sciences
-
Yukuhiro K
National Inst. Agrobiological Sci. Ibaraki Jpn
-
NAGARAJU Javaregowda
Seribiotech Research Laboratory
-
CHAVANCY Gerard
Unite Nationale Sericicole, INRA, La Mulatiere
-
COUBLE Pierre
Centre de Genetique Moleculaire et Cellulaire, CNRS UMR 5536, Universite Lyon
-
Couble Pierre
Centre De Genetique Moleculaire Et Cellulaire Cnrs Umr 5536 Universite Lyon
-
Nagaraju J
Seribiotech Research Laboratory
-
Tamura Toshiki
National Institute Of Sincultural And Entomological Science
-
Chavancy Gerard
Unite Nationale Sericicole Inra La Mulatiere
-
Kanda Toshio
National Institute Of Agrobiological Sciences
関連論文
- Cloning and characterization of a β-N-acetylglucosaminidase (BmFDL) from silkworm Bombyx mori(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Identification of Four Major Hornet Silk Genes with a Complex of Alanine-Rich and Serine-Rich Sequences in Vespa simillima xanthoptera Cameron
- A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm
- Evaluating Promoter Sequences for Trapping an Enhancer Activity in the Silkworm Bombyx mori
- An Improved DNA Injection Method for Silkworm Eggs Drastically Increases the Efficiency of Producing Transgenic Silkworms
- Conservation of fibroin gene promoter function between the domesticated silkworm Bombyx mori and the wild silkmoth Antheraea yamamai
- Development of a New piggyBac Vector for Generating Transgenic Silkworms using the Kynurenine 3-mono Oxygenase Gene
- MUTATIONS IN THE og GENE, A MOLYBDENUM COFACTOR SULFURASE GENE OF THE SILKWORM, BOMBYX MORI(Genetics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- IDENTIFICATION OF W-3 GENE WHOSE MUTATIONS MAKE SILKWORM LARVAL SKIN TRANSLUCENT BECAUSE OF URIC ACID TRANSPORTATIONI DEFICIENCY(Genetics,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm
- Identification of Four Major Hornet Silk Genes with a Complex of Alanine-Rich and Serine-Rich Sequences in Vespa simillima xanthoptera Cameron
- Molecular Cloning and Expression of Two Novel β-N-Acetylglucosaminidases from Silkworm Bombyx mori
- Attempt at Trangenesis of the Silkworm (Bombyx mori L.) by Egg-Injection of Foreign DNA
- Rescue of the Aojuku white-age translucent (w-3^) Bombyx mori mutant by transgenic expression of the wild-type Bmwh3 gene
- カイコにおける繭糸長の選抜効果
- Extensive Sequence Rearrangements and Length Polymorphism in Fibroin Genes in the Wild Silkmoth, Antheraea yamamai (Lepidoptera, Saturniidae)
- Uniform size of leucine-rich repeats in a wild silk moth Saturnia japonica (Lepidoptera Saturniidae) fibroin
- ORGANDY, A NEW MEMBER OF MITES OF THE SILKWORM(Genetics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Nucleotide sequence variation in mitochondrial COI gene among 147 silkworm (Bombyx mori) strains from Japanese, Chinese, European and moltinism classes
- Silk Genes in Wild Silk Moths
- The mitochondrial genome of a stick insect Extatosoma tiaratum (Phasmatodea) and the phylogeny of polyneopteran insects
- Transient expression of chimeric CAT genes injected into early embryos of the domesticated silk worm Bombyx mori.