A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm
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概要
- 論文の詳細を見る
We constructed a new plasmid vector for the production of a modified silk fibroin heavy chain protein (H-chain) in the transgenic silkworm. The plasmid (pHC-null) contained the promoter and the 3′ region of a gene encoding the H-chain and the coding regions for the N-terminal domain and the C-terminal domain of the H-chain. For the model protein, we cloned a foreign gene that encoded EGFP between the N-terminal domain and the C-terminal domain in pHC-null and generated transgenic silkworms that produced a modified H-chain, HC-EGFP. Transgenic silkworms produced HC-EGFP in the posterior part of silk gland cells, secreted it into the lumen of the gland, and produced a cocoon with HC-EGFP as part of the fibroin proteins. N-terminal sequencing of HC-EGFP localized the signal sequence cleavage site to between positions A(21) and N(22). These results indicate that our new plasmid successfully produced the modified H-chain in a transgenic silkworm.
- 社団法人 日本農芸化学会の論文
- 2007-12-23
著者
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Tamada Yasushi
National Institute Of Agrobiological Sciences
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Kojima Katsura
National Institute Of Agrobiological Sciences
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Tamura Toshiki
National Institute of Agrobiological Sciences
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Kojima Katsura
National Inst. Agrobiological Sci. Ibaraki Jpn
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Kobayashi Isao
National Food Research Institute
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KUWANA Yoshihiko
National Institute of Agrobiological Sciences
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Sezutsu Hideki
National Institute of Agrobiological Sciences
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UCHINO Keiro
National Institute of Agrobiological Sciences
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KOBAYASHI Isao
Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences
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Tamada Yasushi
National Inst. Agrobiological Sci. Ibaraki Jpn
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Kojima Katsura
Silk-materials Research Unit National Institute Of Agrobiological Sciences
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Tamada Yasushi
Insect Biomaterial And Technol. Dep. National Inst. Of Agrobiological Sci. (nias)
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Uchino Keiro
Transgenic Silkworm Research Center National Institute Of Agrobiological Sciences
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Tamura Toshiki
Division Of Insect Sciences National Institute Of Agrobiological Sciences
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Kobayashi Isao
Transgenic Silkworm Research Center National Institute Of Agrobiological Sciences
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Kobayashi Isao
National Food Res. Inst. Ibaraki Jpn
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Sezutsu Hideki
Transgenic Silkworm Research Unit Genetically Modified Organism Research Center National Institute Of Agrobiological Sciences
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