HuR Knockdown Changes the Oncogenic Potential of Oral Cancer Cells
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概要
- 論文の詳細を見る
HuR binds to AU-rich element (ARE) containing mRNA to protect them from rapid degradation. Here, we show that knockdown of HuR changes the oncogenic properties of oral cancer cells. Oral squamous cell carcinoma cell lines, HSC-3 and Ca9.22, which express HuR protein and cytoplasmic ARE-mRNA more abundantly than normal cells, were subjected to HuR knockdown. In the HuR-knockdown cancer cells, the cytoplasmic expression of c-fos, c-myc, and COX-2 mRNAs was inhibited compared to those in cells that had been transfected with a control siRNA, and the half-lives of these mRNAs were shorter than those of their counterparts in the control cells. HuR-knockdown cells failed to make colonies in soft agar, suggesting that the cells had lost their ability for anchorage-independent cell growth. Additionally, the motile and invasive activities of the cells decreased remarkably by HuR knockdown. Furthermore, the expression of cell cycle-related proteins, such as cyclin A, cyclin B1, cyclin D1, and CDK1, was reduced in the HuR-knockdown cancer cells, and HuR bound to cdk1 mRNA in order to stabilize it. These findings suggest that HuR knockdown changes the features of oral cancer cells, at least in part, by affecting their cell cycle, and shows potential as an effective therapeutic approach.
- American Association for Cancer Researchの論文
著者
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Kitagawa Yoshimasa
Oral Diagnosis And Medicine Department Of Oral Pathobiological Science Graduate School Of Dental Med
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Kitagawa Yoshimasa
北海道大学 歯学研究科口腔病態学口腔診断内科学
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Higashino Fumihiro
Department Of Oral Pathology And Biology Division Of Oral Pathobiological Science Hokkaido Universit
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Kitamura Tetsuya
Department Of Oral Pathology & Biology Hokkaido University Graduate School Of Dental Medicine
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Higashino Fumihiro
Department Of Oral Pathology & Biology Hokkaido University Graduate School Of Dental Medicine
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Shindoh Masanobu
Division Of Oral Pathobiological Science Graduate School Of Dental Medicine Hokkaido University
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Shindoh Masanobu
Department Of Oral Pathology And Biology Hokkaido University Graduate School Of Dental Medicine
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Shindoh Masanobu
Department Of Oral Pathology And Biology Division Of Oral Pathobiological Science Hokkaido Universit
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Totsuka Yasunori
Oral And Maxillofacial Surgery Department Of Oral Pathobiological Science Graduate School Of Dental
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