Molecular Characterization of a New Begomovirus Infecting Honeysuckle in Kobe,Japan

概要

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A virus (HY12) isolated from honeysuckle (Lonicera japonica Thunb.) collected in Kobe,Hyogo prefecture, Japan, showing symptoms of yellow vein mosaic, was characterized. HY12 was transmitted to Nicotina benthamiana by sap inoculation while the other honeysuckle isolates could not. The complete DNA-A and DNA-β like molecules of the isolate were determined and found to comprise 2763 and 1262 nucleotides (nt), respecrively, with a typical begomovirus genome organization. The DNAβ molecule had the conserved ORF (βC1) and satellite conserved regioin (SCR) typical of the Geminiviridae family containing a 5'-TAATATTAC-3' motif in the stem loop and the adenine-(A) rich region. Additionally, its entire sequence was interrupted at intervals with sequences revealed that were not viral and whose origin was unknown. A comparison of the DNA-A sequences revealed that HY12 shared 74% with four other honeysuckle begomoviruses but 88-89% with tobacco begomoviruses found in Japan. The isolate shared up to 92% of the IR sequences with the honeysuckle begomoviruses but only 60-88% with those of tobacco. Ohylogenetic analysis of full length DNA-A component as well as its CP clusterd HY12 with the tobacco begomoviruses. HY12 is an isolate of Honeysuckle yellow vein mosaic virus.
2005-02-01