Microsatellite-centromere mapping in the Japanese eel (Anguilla japonica) by half-tetrad analysis using induced triploid families
スポンサーリンク
概要
- 論文の詳細を見る
Genetic improvement of the Japanese eel (Anguilla japonica) can be achieved by artificially controlling its life cycle using recent advances in reproductive biology. In this study, we developed 43 microsatellite loci to confirm Mendelian inheritance at 10 of them as well at 16 previously reported in two full-sib families produced by artificial insemination. In order to establish a base for aquaculture genetics of this species in the near future, these microsatellite loci were mapped in relation to the centromere by half-tetrad analysis using four artificially induced triploid families. The second division segregation frequency (y) of the microsatellite loci ranged from 0.008 to 0.968 (mean ± SD = 0.645 ± 0.298). These results suggest the presence of strong chiasma interference in the eel. Significant differences were observed for the map distances of microsatellite loci between the two isolation procedures. Microsatellites isolated using the enrichment procedure were mapped to various sites starting from the centromere to the telomere, whereas those from the conventional size-selected library showed a tendency to be distributed in the telomeric region.
- Elsevierの論文
- 2006-06-30
著者
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Tanaka Hideki
National Research Institute of Aquaculture
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Okubo K
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
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Okubo Kataaki
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
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Tanaka H
National Research Institute Of Aquaculture
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Okuzawa K
National Research Institute Of Aquaculture
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Ohta Hiromi
Department Of Fisheries Graduate School Of Agriculture Kinki University
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Ohta Hiromi
Department Of Fisheries Kinki University
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Ohta H
Department Of Fisheries Kinki University
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Tanaka Hideji
National Research Institute Of Aquaculture
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Okumura H
Division Of Marine Biosciences Graduate School Of Fisheries Sciences Hokkaido University
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