HBs抗原染色の改良法と染色機構

概要

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HBs Ag in the hepatocytes have been stained by orcein methods. However, not only HBs Ag but also degenerative and necrotic cytoplasm of hepatocytes are occasionally stained by the method, that makes it difficult to identify HBs Ag. Moreover, orcein dyes have different staining properties from lots to lots, that causes difficulty in obtaining a stable result. Therefore, we tried to modify the orcein method and found that the procedure using a sensitizer (ferric ammonium sulfate or uranium nitrate) after oxidizing solution gave stable and satisfactory results. By this method, in the hepatocytes, only HBs Ag are stained positively. The mechanism of function of the sensitizer is not clear, but we considered that Fe or U ions occupy small gaps in the protain molecular structure in the cells before orcein molecules occupy these gaps and that the method does not give a false positive result. It is also suggested that various HBs-staining-dyes can react with SO_3H groups which are formed by the oxidization of SS groups of proteins. We have developed a new staining method for HBs Ag, using Resorcin-fuchsin. The results of our method were also discussed.
長崎大学熱帯医学研究所,Institute of Tropical Medicine, Nagasaki Universityの論文
1980-10-30