アカイエカ群の1系統におけるテトラサイクリンによるWolbachia pipientisの駆除と和合性の変化

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A wild type strain of Culex pipiens complex, which originated from Scauri, Italy, and is maintained at the Division of Epidemiology, School of Public Health, University of California, Los Angeles, was cured by exposing first-inster larvae to a 2.8, 3.6, and 5.0% solution of Tetracycline Hydrochloride in distilled water for 16-20 hours before food (rabbit pellets) was added. Thereafter, the solution was exchanged for fresh water and food was added daily. In lines B, C, and D, which were treated with 2.8% (34 C), 3.6% (34 C), and 2.8% (24 C) solution, a few microorganisms were found from the gonads at the third generation after the treatment, and it was shown that these two concentration were not enough for curing of Wolbachia. Only E line, 5.0% concentration (34 C), eradicated infection in the larvae tested, as shown by examination of smears of gonads stained with Giemsa stain. Aposymbiotic (symbiote-free) line was produced in this way, and this strain remained free of Wolbachia in subsequent generations. Although treated and aposymbiotic females produced viable progeny when mated with treated or aposymbiotic males, the females produced no progeny when back crossed with the original males with symbiotes. The results of this experiment support the hypothesis of Yen and Barr (1971, 1973) that Wolbachia might be the cause of cytoplasmic incompatibility, and the males "cured" of their Wolbachia infections no longer manifested incompatibility.
長崎大学熱帯医学研究所,Institute of Tropical Medicine, Nagasaki Universityの論文
1982-03-31

アカイエカ群の1系統におけるテトラサイクリンによるWolbachia pipientisの駆除と和合性の変化

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