New scaffolds for tissue engineering using adipose-derived stem cells

概要

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Adipose-derived stem cells (ADSCs) have attracted attention as a useful cell source for tissue engineering, even in the clinical setting. However, it is difficult to regenerate tissues with complex structures in the absence of a three-dimensional matrix such as an artificial scaffold. Namely, scaffolds are required to allow implanted ADSCs to regenerate tissues with three-dimensional structures. Although non-woven PGA (poly (glycolic acid)) fabric has been used widely as an artificial scaffold, ADSCs poorly adhere to and grow on PGA fabric due to its hydrophobicity. To improve cell adhesion and growth on PGA fibers, we devised a PGA fabric coated with collagen. We isolated ADSCs from the inguinal adipose tissue of a rat and characterized the cells using flow cytometry with both CD29- and CD90-positive markers and CD11b- and CD45-negative markers. Our cell growth examination revealed that ADSC growth was improved on all of the collagen-coated fabrics compared with that observed on the corresponding non-coated fabrics. This may be because ADSCs are able to adhere and grow more readily on the hydrophilic surface of collagen-coated PGA fabrics.
2013-04-00