The effects of thermally cross-linked gelatin film on intraperitoneal dissemination of cancer cells : an in vitro study using human gastrointestinal cancer cell lines
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概要
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To prevent adhesion formation after abdominal surgery, we newly developed a thermally cross-linked gelatin film and previously reported its superior anti-adhesive effects with excellent peritoneal regeneration. However, since the gelatin film is made from a degenerated product of collagen which is the most abundant extracellular matrix protein in mammals and has been used as a scaffold in the field of regeneration medicine, it may act as a scaffold convenient for cancer cell growth, thereby accelerating peritoneal dissemination when used in surgery for abdominal cancers. To clarify this issue, in this study, we examined effects of the gelatin film on in vitro cancer cell growth, using the human colon cancer cell line HT29 and the human gastric cancer cell lines MKN74 and KATO-III. HT29 and MKN74 cells exhibit an adherent growth mode and Kato-III cells exhibit a semi-floating growth mode in conventional culturing. The cancer cells were cultured on the gelatin film or without the film (control), and each viable cell number was counted at 1, 3, 5 and 7 days after seeding. The growth of the HT29 and MKN74 cells was significantly less than that observed in the control group, although both cells grew on the gelatin film with time. Compared with the other two cell lines, the KATO-III cells seemed to grow on the gelatin films well. However, the cell growth was also less than that observed in the control group. The results of this in vitro study suggest that gelatin film may not act as a scaffold convenient for cancer cell growth or accelerate peritoneal dissemination, although further in vivo studies are needed.
- 2013-04-00
著者
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Tsujimoto Hiroyuki
Department Of Biotechnology Faculty Of Engineering Kansai University
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Urabe Mamoru
Department Of Obstetrics And Gynecology Kyoto Prefectural University Of Medicine
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Hagiwara Akeo
Department Of Digestive Surgery Kyoto Prefectural University Of Medicine
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Takamori Hideki
Department Of Chemistry And Materials Science Tokyo Institute Of Technology
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Torii Hiroko
Graduate Student Department Of Medical Life System Doshisha University
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Morita Shinichiro
Research and Development Department, Gunze Ltd
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Torii Hiroko
Graduate Student, Department of Medical Life System, Doshisha University/Kusatsu General Hospital
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Ozamoto Yuki
Kusatsu General Hospital
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Miyamoto Hiroe
Undergraduate Sophomore, Department of Medical Life System, Doshisha University
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Horii Tsunehito
Undergraduate Sophomore, Department of Medical Life System, Doshisha University
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Ikeda Junki
Graduate Student, Department of Medical Life System, Doshisha University
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Orikasa Taichi
Graduate Student, Department of Medical Life System, Doshisha University
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Takamori Hideki
Department of Medical Life System, Doshisha University/Research and Development Department, Gunze Ltd
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Urabe Mamoru
Department of Medical Life System, Doshisha University/Department of Kusatsu General Hospital
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Tsujimoto Hiroyuki
Department of Medical Life System, Doshisha University
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