微生物を利用した土壌病害の防除(環境と土壌微生物,シンポジウム)

概要

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Bottle gourd, a cucurbitaceae, plant has been cultivated for 300 years, and is planted on 2,500 ha. annually in Tochigi Pref. Fusarium wilt of bottle gourd caused by Fusarium oxysporum f sp. lagenariae is serious. In many commercial fields, welsh onion, a Allium fistulosum has been mixed-cropped, as an associated crop with bottle gourd. Burkholderia glagioli was isolated frequently in associated with root of welsh onion, and isolates showed antifungal activity to F. oxysporum f sp. lagenariae. The antifungal bacteria grow on the roots of welsh onion and chinese chive, and produces antifungal substans, a Pyrrolnitrin which are spread into the soil. Fungi and bacteria do not exist in interior tissues of healthy plants, except for Sweet potato and Cyclamen. Microoganisms frequently isolated from interior tissue of these plants, and the isolates easily associate with interior tissues of plants. To confirm if this phenomenon also occurs in other dicotyledon plant, the seedlings of these plants were cut off at middle of the hypocotyl in 2 to 3 leaves stage and cut of the plants were soaked into the bacteria suspension. After inoculation the cuttings were planted in propagation beds(=hypocotyl cutting inoculation). Isolates colonized in vessel and inter cellular space of 32 species dicotyledon plants.
日本土壌微生物学会の論文
1998-10-01