Jatoba (Hymenaea courbaril L.)果皮からのチロシナーゼ活性阻害物質

概要

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Jatoba (Hymenaea courbaril) pericarp was extracted with 70% aqueous acetone after defatting with petroleum ether and n-hexane. The extract was separated by sephadex LH-20 column chromatography using water, ethanol, methanol, and 70% aqueous acetone, sequentially, as eluents. The methanol and 70% aqueous acetone fractions (ME and AE, respectively) showed high tyrosinase inhibitory effects in vitro, and suppressed melanin formation in B-16 mouse melanoma cell line. But this suppression was attributable to the inhibition of the cell growth. Eighty to ninety percent of these fractions were flavanols according to the analysis by the Folin-Denis and Vanillin-hydrochloric acid methods. Phenyl nucleus analysis of proanthocyanidins (PAC) of these fractions resulted in a procyanidin type, 5,7,3,4-flavan-3-ol unit. The avarage degree of polymerization of PAC in ME and AE were shown to be five and twelve, respectively, by gel permeation chromatography. The tyrosinase inhibitory activity increased with the increase of molecular weight of PAC.
日本生薬学会の論文
1999-02-20