イオンペアークロマトグラフィーによる呉茱萸配合漢方エキス製剤中のSynephrineの定量

概要

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A simple and reliable ion-pair HPLC method was established for the determination of synephrine contents in kampo prescriptions containing Evodiae Fructus, by using sodium dodecyl sulfate (SDS) as an ion-pair reagent. The best procedure for extracting synephrine was to treat kampo medicines with H_2O-CH_3CN-SDS-H_3PO_4 (73 : 27 : 0.5 : 0.1) with stirring for 10 min. When an ODS column and SDS as an ion-pair reagent were used, synephrine was eluated within 31 min without interference from co-existing components. By using this method, kampo extract preparations containing Evodiae Fructus extract were examined for their synephrine contents. The results revealed that the synephrine contents in such kampo extract preparations were 8-13% of those in the corresponding pharmaceutical decoctions reported previously.
日本生薬学会の論文
1995-12-20