イオンペアー高速液体クロマトグラフィーによる牡丹皮配合漢方湯中のPaeonolの定量

概要

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A simple and reliable method was established for quantitative determination of paeonol in oriental decoctions containing Moutan bark. This method using high-performance liquid chromatography with 1OmM tetra-n-amylammonium bromide (as an ion-pair reagent )-CH_3CN (75 : 25,pH6.8) and an ODS column, and gives better separation of paeonol than the previous phosphate buffer method or the ion-suppression method. This method was applied to 15 kinds of oriental pharmaceutical decoctions containing Moutan bark. From 13 of them, paeonol was detected within 30 min without interference from co-existing components. The other two decoctions, i.e. Kamishouyou- san-go-shimtsu-to and Keishibukuryo-gan, however, needed separation by a gradient method.
日本生薬学会の論文
1998-04-20