イオンペアー高速液体クロマトグラフィーによる半夏厚朴湯の主要成分の同時定量

概要

論文の詳細を見る
A simple method was established for the precise simultaneous determination of five marker components in the oriental pharmaceutical decoction "Hangekoboku-to" and the relevant Kampo extract preparations. The five components, 3,4-dihydroxy-benzaldehyde, rosmarinic acid, honokiol, magnolol and 6-gingerol, were separated by high-performance liquid chromatography using an ODS column with gradient elution system of 10mM tetra-n-butylammonium phosphate (low UV type, pH 4.0)-methanol in which the methanol content was liniearly increased from 10% to 60%. These five components were eluted within 60min without interference from co-exisiting components. This method was compared with three other methods, a water-methanol method, an ion-suppression method, and a phosphate buffer method.
日本生薬学会の論文
1999-10-20