イオンペアー高速液体クロマトグラフィーによる漢方湯液"四逆散"の主要成分の同時定量

概要

論文の詳細を見る
A simple method was established for the precise simultaneous determination of four marker components in the oriental pharmaceutical decoction "Shigyaku-san." These four components, paeoniflorin, hesperidin, glycyrrhizin and saikosaponin A, were separated by HPLC using an ODS column with a gradient elution system of 5 mm tetra-n-amylammonium bromide (pH 4.0)-acetonitrile in which the acetonitrile content was linearly increased from 10 to 80%. The four marker components were eluted within 75 min without interference from co-existing components. This method was compared with four known methods, i.e. the water-acetonitrile method, ion-pair method using tetra-n-butylammonium hydroxide as an ion-pair reagent, ion-suppression method and phosphate buffer method.
日本生薬学会の論文
1997-06-20