クリーンアナリシスを指向したゴオウ中のビリルビン定量法

概要

論文の詳細を見る
BEZOAR BOVIS is a calculus composed of bilirubin in the gall bladder of cows, which is listed in the Japanese pharmacopeia fifteenth edition (JP15). It is important to determine the quantity of bilirubin in BEZOAR BOVIS by evaluating the quality of the BEZOAR BOVIS. Although a rough measuring method for the amount of ingredients has been reported, a quantitative analytical method for bilirubin is not described in JP15. The conventional method is not reliable, because many contaminants besides bilirubin affect the quantitative value of BEZOAR BOVIS. Furthermore, it is desirable to develop a clean analysis because chloroform is always used as the solvent for measuring the amount of ingredients and for the conventional quantitative methods comprising HPLC and colorimetric techniques. In the present study we developed an effective and clean method, in which BEZOAR BOVIS was extracted with DMSO instead of chloroform and the resulting extract was directly analyzed using HPLC. This established method is a prompt and reliable quantitative method and can be used to examine the amount of bilirubin in the BEZOAR BOVIS with good precision, specificity and linearity. Further, it was found that this method showed good agreement with the conventional quantitative methods in terms of their quantitative values.
2010-08-20