逆相TLC/スキャニングデンシトメトリーによる生薬分析 : ヤクチ,インヨウカク,シコン,ダイオウ,モッコウ,クジンの確認試験

概要

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We performed identification tests of the main components (nootkatone, icariin, acetylshikonin, sennoside A, costunolide, and oxymatrine) of the following crude drugs in the Japanese pharmacopoeia, 15th edition (JP15): Bitter Cardamon, Epimedium Herb, Lithospermum Root, Rhubarb, Saussurea Root, and Sophora Root. The identification was carried out with reversed-phase TLC using water, acetonitrile, methanol, and 2-butanone as developing solvents. A single spot could be separated from other components (Rf value: 0.43-0.61). In addition, spectral information could be simultaneously obtained by scanning densitometry. Thus, the main components of crude drugs in the JP15 could be identified by this method simply, rapidly, and accurately.
日本生薬学会の論文
2009-02-20