Statistical optimization of culture conditions for the production of enniatins H, I, and MK1688 by Fusarium oxysporum KFCC 11363P(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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The aim of this study was to optimize the culture conditions for the production of biological cyclic hexadepsipeptides (enniatins H, I and MK1688) from Fusarium oxysporum KFCC 11363P. Tests of 10 complete or chemically defined liquid culture media revealed that Fusarium defined medium was the best for the production of enniatins (produced amounts: enniatin H, 185.4mg/L; enniatin I, 349.1mg/L; enniatin MK1688, 541.1mg/L; and total enniatins, 1075.6mg/L). On the eighth day after inoculation, the maximal production of enniatins was observed at 25℃ in Fusarium defined medium. The optimal carbon and nitrogen sources for producing biological cyclic hexadepsipeptides (enniatins H, I, and MK1688) were sucrose and NaNO_3, respectively, and their optimal concentrations were determined by the principle of response surface methodology. It was confirmed that using the optimized growth medium compositions increased the amounts of enniatins H, I, and MK1688, and total enniatins produced to 695.2, 882.4, 824.8, and 2398.5mg/L, respectively. These findings will assist in formulating microbiological media useful for enniatin research.
著者
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Lee Chan
Department Of Chemical And Biological Engineering Korea University
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Park Sang-gyu
Department Of Statistics Chung-ang University
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Kim Byung
Department Of Food Science And Technology Bet Research Institute Chung-ang University
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Park Sang-gyu
Department Of Electronic Engineering Hanyang University
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Lee Hee-seok
Department Of Food Science And Technology Bet Research Institute Chung-ang University
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Kang Jea-wook
Department Of Food Science And Technology Bet Research Institute Chung-ang University
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Lee Chan
Department Of Food Science And Technology Bet Research Institute Chung-ang University
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Kim Byung
Department Of Aerospace Engineering School Of Engineering Nagoya University
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LEE Chan
Department of Food Science and Technology, BET Research Institute, Chung-Ang University
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